Document Detail

Differentiation of neural cells in the fetal cerebral cortex of cynomolgus monkeys (Macaca fascicularis).
MedLine Citation:
PMID:  22330652     Owner:  NLM     Status:  MEDLINE    
Proliferation and programmed cell death are important in the formation of morphologic structures and functional activity during CNS development. We used immunohistochemical and TUNEL methods to examine the proliferation and differentiation of neural cells in, distribution of apoptotic cells in, and microglial cell involvement in the removal of apoptotic cells from the fetal cerebral cortex of cynomolgus monkeys. At embryonic day (E) 50 and E80, the neuroepithelium contained many mitotic cells. Cells staining for PCNA (a nuclear marker of proliferating cells) were prominent in the proliferative zone, whereas cells positive for NeuN (a neuron-specific marker) were absent. GFAP staining for glial cells was positive in the neuroepithelium and radial glial fibers. Iba1-positive cells (that is, macrophages and microglia) were distributed throughout all regions at all time points but accumulated especially in the ventricular zone at E80. Apoptotic morphology (at E80) and TUNEL-positive cells (that is, containing DNA fragmentation; at E50 and E80) were observed also. At E120 and E150, most PCNA-positive cells were in the ventricular zone, and NeuN-positive cells were prominent in all layers except layer I-II at E120. GFAP immunoreactivity was detected mainly in cells with fine processes in the white matter. Neither apoptosis nor TUNEL-positive cells were detected at either E120 or E150. These results suggest that proliferation, migration, and neural cell death occur during midgestation (that is, E50 to E80) in fetal brain of cynomolgus macaques, whereas differentiation and maturation of neural cells occur after midgestation (E80).
Yujiro Toyoshima; Satoshi Sekiguchi; Takayuki Negishi; Shinichiro Nakamura; Toshio Ihara; Yoshiyuki Ishii; Shigeru Kyuwa; Yasuhiro Yoshikawa; Kimimasa Takahashi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative medicine     Volume:  62     ISSN:  1532-0820     ISO Abbreviation:  Comp. Med.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-14     Completed Date:  2012-06-21     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  100900466     Medline TA:  Comp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  53-60     Citation Subset:  IM    
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
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MeSH Terms
Cell Differentiation / physiology*
Cerebral Cortex / cytology*,  embryology
Fetus / embryology*
Immunohistochemistry / veterinary
In Situ Nick-End Labeling / veterinary
Macaca fascicularis / embryology*
Neurons / physiology*
Proliferating Cell Nuclear Antigen / metabolism
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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