Document Detail

Differentiation of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus by polymerase chain reaction.
MedLine Citation:
PMID:  10499296     Owner:  NLM     Status:  MEDLINE    
Lactobacillus casei, Lact. paracasei and Lact. rhamnosus form a closely related taxonomic group within the heterofermentative lactobacilli. These three species are difficult to differentiate using traditional fermentation profiles. We have developed polymerase chain reaction primers which are specific for each of these species based on differences in the V1 region of the 16S rRNA gene. Sixty-three Lactobacillus isolates from cheese were identified using these primers. The 12 Lact. rhamnosus and 51 Lact. paracasei identified in this way were also differentiated using a randomly amplified polymorphic DNA (RAPD) primer.
L J Ward; M J Timmins
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  29     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-10-14     Completed Date:  1999-10-14     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  90-2     Citation Subset:  IM    
New Zealand Dairy Research Institute, Palmerston North, New Zealand.
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MeSH Terms
Bacterial Typing Techniques
Base Sequence
Cheese / microbiology
Lactobacillus / classification*,  genetics
Molecular Sequence Data
Polymerase Chain Reaction*
RNA, Bacterial / analysis
RNA, Ribosomal, 16S / analysis
Random Amplified Polymorphic DNA Technique
Sequence Alignment
Species Specificity
Reg. No./Substance:
0/RNA, Bacterial; 0/RNA, Ribosomal, 16S

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