Document Detail

Differentiation of E14 mouse embryonic stem cells into thyrocytes in vitro.
MedLine Citation:
PMID:  19886789     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: If methods of differentiating stem cells into thyrocytes can be perfected, they may provide a ready source of normal thyrocytes for basic research and clinical application. We developed a novel culture method capable of differentiating mouse embryonic stem (ES) cells into thyroid follicular cells. METHODS: E14 mouse ES cells were allowed to differentiate into embryoid bodies and then stimulated with thyroid-stimulating hormone, insulin, and potassium iodide. The resulting differentiated cells were observed for expression of thyrocyte-specific mRNA transcripts with reverse transcriptase (RT)-polymerase chain reaction. To definitively identify thyrocytes, we simultaneously observed the thyrocyte-specific proteins, thyroid transcription factor-1 and PAX-8, with dual-color immunofluorescent labeling. The cells were further characterized by electron microscopy. RESULTS: The ES cells were successfully differentiated into thyrocytes. Differentiated cells expressed PAX-8, thyroid-stimulating hormone receptor, sodium/iodide symporter, thyroperoxidase, and thyroglobulin mRNAs, and coexpressed thyroid transcription factor-1 and PAX-8 proteins. The extent of differentiation was further explored by electron microscopy, which showed that differentiated cells had ultrastructural features similar to adult human thyrocytes, whereas the cells from unstimulated cultures were mostly disintegrated and lacked developed organelle structures. CONCLUSIONS: These data show that E14 mouse ES cells can be differentiated into thyrocytes by culturing with thyroid-stimulating hormone, insulin, and potassium iodide. The development of reliable methods to produce thyroid cells from ES cells is important to future research in thyroid biology and medical applications.
Ningyi Jiang; Yingying Hu; Xiongying Liu; Yanfeng Wu; Hong Zhang; Guibing Chen; Jiugen Liang; Xianping Lu; Sheng Liu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thyroid : official journal of the American Thyroid Association     Volume:  20     ISSN:  1557-9077     ISO Abbreviation:  Thyroid     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-13     Completed Date:  2010-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9104317     Medline TA:  Thyroid     Country:  United States    
Other Details:
Languages:  eng     Pagination:  77-84     Citation Subset:  IM    
Department of Nuclear Medicine, The Second Affiliated Hospital of Sun Yat-Sen University, GuangZhou, China.
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MeSH Terms
Cell Communication
Cell Differentiation / drug effects*
Coculture Techniques
Embryonic Stem Cells / cytology*,  drug effects,  ultrastructure
Insulin / pharmacology
Microscopy, Confocal
Microscopy, Fluorescence
Nuclear Proteins / genetics,  metabolism
Paired Box Transcription Factors / genetics,  metabolism
Potassium Iodide / pharmacology
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Thyroid Gland / cytology*,  ultrastructure
Thyrotropin / pharmacology
Transcription Factors / genetics,  metabolism
Reg. No./Substance:
0/Nuclear Proteins; 0/Paired Box Transcription Factors; 0/Pax8 protein, mouse; 0/RNA, Messenger; 0/Transcription Factors; 0/thyroid nuclear factor 1; 11061-68-0/Insulin; 7681-11-0/Potassium Iodide; 9002-71-5/Thyrotropin
Comment In:
Thyroid. 2010 Jan;20(1):1-2   [PMID:  20067376 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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