| Differentiating short- and long-term effects of diet in the obese mouse using 1H-NMR metabolomics. | |
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MedLine Citation:
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PMID: 21486381 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This study determined if targeted (1) H-NMR metabolomic profiling of serum could be employed to distinguish the effects of obesity from those of diet in mice. Following weaning, littermates were randomly divided into two diet groups; chow or high fat. After 12wk of dietary manipulation, fat-fed animals were obese and hyperglycaemic. Mice from each treatment either maintained their current diet, or switched to the opposite diet for a final week. Differences in metabolite levels were determined using Orthogonal Projection to Latent Structures and cross-validated discriminant analysis. The short and long-term effects of each diet could be clearly distinguished. Short-term diet effects are the major contributor to the metabolic profile, underscoring the need for controls beyond the standard fast before serum collection. This work demonstrates the importance of dietary controls when attempting to isolate obesity related changes, and highlights the ability of metabolomics to identify subtle changes when experiments are properly structured. |
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Authors:
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Gavin Duggan; Dustin S Hittel; Curtis C Hughey; Aalim Weljie; Hans J Vogel; Jane Shearer |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-12 |
Journal Detail:
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Title: Diabetes, obesity & metabolism Volume: - ISSN: 1463-1326 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883645 Medline TA: Diabetes Obes Metab Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Blackwell Publishing Ltd. |
Affiliation:
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Department of Biological Sciences, Bio-NMR Center, Faculty of Science Faculty of Kinesiology Department of Biochemistry and Molecular Biology, Faculty of Medicine. University of Calgary, Calgary, AB. T2N 4N1. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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