Document Detail


Differentially expressed nucleolar TGF-beta1 target (DENTT) shows tissue-specific nuclear and cytoplasmic localization and increases TGF-beta1-responsive transcription in primates.
MedLine Citation:
PMID:  15823505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differentially Expressed Nucleolar TGF-beta1 Target (DENTT) is a new member of the TSPY/TSPY-like/SET/NAP-1 (TTSN) superfamily whose mRNA is induced by TGF-beta1 in TGF-beta1-responsive human lung cancer cells. Monkey DENTT mRNA contains a 2085-bp open reading frame that encodes a predicted polypeptide of 695 amino acids with five nuclear localization signals, two coiled-coil regions, and a domain that shows significant identity to a region that defines the TTSN superfamily. RT-PCR amplification and Western blot analyses showed DENTT mRNA and protein in adult monkey tissues, including the adrenal gland, cerebral cortex, and ovary. Immunohistochemical staining showed that numerous neurons were intensely immunoreactive for DENTT, as were anterior pituitary secretory cells, thyroid follicular cells, and smooth muscle cells of arteries and lung bronchial walls. DENTT expression was also prominent in monkey bronchiolar-alveolar adenomas and cell lines. While the addition of TGF-beta1 or retinoic acid to monkey normal lung bronchial 12MBr6 cells and human lung cancer NCI-H727 cells increased DENTT protein production, TGF-beta1 together with retinoic acid resulted in a more sustained increase in DENTT production than with TGF-beta1 or retinoic acid alone. Transient transfection studies showed that ectopic DENTT expression significantly increased TGF-beta1-responsive 3TP-Lux and CAGA12-Lux reporter transcription in 12MBr6 and NCI-H727 cells with TGF-beta1 addition, while ectopic DENTT expression had no significant effect on the transcription of a retinoic acid-responsive element reporter in the presence of retinoic acid or TGF-beta1. These findings suggest new possibilities for DENTT as a TGF-beta1-regulated, but not a retinoic acid-regulated member of the TTSN superfamily in primate physiology.
Authors:
Laurent L Ozbun; Alfredo Martínez; Sonia B Jakowlew
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-03-22
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1728     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-28     Completed Date:  2005-06-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  163-80     Citation Subset:  IM    
Affiliation:
National Cancer Institute, Cell and Cancer Biology Branch, 9610 Medical Center Drive, Suite 300, Rockville, MD 20850, USA.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / metabolism
Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Blotting, Western
Cell Line
Cell Nucleus / metabolism*
Cercopithecus aethiops
Cerebral Cortex / metabolism
Cytoplasm / metabolism*
DNA Primers
Disease Models, Animal*
Female
Humans
Immunohistochemistry
Lung / metabolism
Lung Neoplasms / genetics*
Macaca / genetics*
Molecular Sequence Data
Myocytes, Smooth Muscle / metabolism
Nuclear Proteins / genetics,  metabolism*
Ovary / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology
Thyroid Gland / metabolism
Transcription, Genetic / drug effects*,  genetics
Transforming Growth Factor beta / genetics,  metabolism*,  pharmacology
Transforming Growth Factor beta1
Tretinoin / pharmacology
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Nuclear Proteins; 0/SE20-4 protein, human; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 302-79-4/Tretinoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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