|Differentially expressed nucleolar TGF-beta1 target (DENTT) shows tissue-specific nuclear and cytoplasmic localization and increases TGF-beta1-responsive transcription in primates.|
|PMID: 15823505 Owner: NLM Status: MEDLINE|
|Differentially Expressed Nucleolar TGF-beta1 Target (DENTT) is a new member of the TSPY/TSPY-like/SET/NAP-1 (TTSN) superfamily whose mRNA is induced by TGF-beta1 in TGF-beta1-responsive human lung cancer cells. Monkey DENTT mRNA contains a 2085-bp open reading frame that encodes a predicted polypeptide of 695 amino acids with five nuclear localization signals, two coiled-coil regions, and a domain that shows significant identity to a region that defines the TTSN superfamily. RT-PCR amplification and Western blot analyses showed DENTT mRNA and protein in adult monkey tissues, including the adrenal gland, cerebral cortex, and ovary. Immunohistochemical staining showed that numerous neurons were intensely immunoreactive for DENTT, as were anterior pituitary secretory cells, thyroid follicular cells, and smooth muscle cells of arteries and lung bronchial walls. DENTT expression was also prominent in monkey bronchiolar-alveolar adenomas and cell lines. While the addition of TGF-beta1 or retinoic acid to monkey normal lung bronchial 12MBr6 cells and human lung cancer NCI-H727 cells increased DENTT protein production, TGF-beta1 together with retinoic acid resulted in a more sustained increase in DENTT production than with TGF-beta1 or retinoic acid alone. Transient transfection studies showed that ectopic DENTT expression significantly increased TGF-beta1-responsive 3TP-Lux and CAGA12-Lux reporter transcription in 12MBr6 and NCI-H727 cells with TGF-beta1 addition, while ectopic DENTT expression had no significant effect on the transcription of a retinoic acid-responsive element reporter in the presence of retinoic acid or TGF-beta1. These findings suggest new possibilities for DENTT as a TGF-beta1-regulated, but not a retinoic acid-regulated member of the TTSN superfamily in primate physiology.|
|Laurent L Ozbun; Alfredo Martínez; Sonia B Jakowlew|
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|Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-03-22|
|Title: Biochimica et biophysica acta Volume: 1728 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2005 May|
|Created Date: 2005-04-28 Completed Date: 2005-06-17 Revised Date: 2006-11-15|
Medline Journal Info:
|Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands|
|Languages: eng Pagination: 163-80 Citation Subset: IM|
|National Cancer Institute, Cell and Cancer Biology Branch, 9610 Medical Center Drive, Suite 300, Rockville, MD 20850, USA.|
|APA/MLA Format Download EndNote Download BibTex|
Amino Acid Sequence
Cell Nucleus / metabolism*
Cerebral Cortex / metabolism
Cytoplasm / metabolism*
Disease Models, Animal*
Lung / metabolism
Lung Neoplasms / genetics*
Macaca / genetics*
Molecular Sequence Data
Myocytes, Smooth Muscle / metabolism
Nuclear Proteins / genetics, metabolism*
Ovary / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Thyroid Gland / metabolism
Transcription, Genetic / drug effects*, genetics
Transforming Growth Factor beta / genetics, metabolism*, pharmacology
Transforming Growth Factor beta1
Tretinoin / pharmacology
|0/DNA Primers; 0/Nuclear Proteins; 0/SE20-4 protein, human; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 302-79-4/Tretinoin|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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