Document Detail


Differential use of endoplasmic reticulum membrane for phagocytosis in J774 macrophages.
MedLine Citation:
PMID:  15753287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sustained phagocytosis requires the continuous replacement of cell-surface membrane from intracellular sources. Depending on the nature of the engulfed particles, a variety of endocytic compartments have been demonstrated to contribute membranes needed for the formation of phagosomes. It has recently been reported that the endoplasmic reticulum (ER) can also fuse with the plasma membrane during phagocytosis [Gagnon, E., Duclos, S., Rondeau, C., Chevet, E., Cameron, P. H., Steele-Mortimer, O., Paiement, J., Bergeron, J. J. & Desjardins, M. (2002) Cell 110, 119-131]. However, there is currently no known mechanistic basis for this fusion process to occur. Here we report that direct ER-plasma membrane fusion during phagocytosis requires the ER resident soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein ERS24/Sec22b and that J774-macrophages react toward the challenge of large (3.0-microm) but not small (0.8-microm) particles by triggering this fusion mechanism, allowing them to access the most abundant endogenous membrane source in the cell, the ER.
Authors:
Thalia Becker; Allen Volchuk; James E Rothman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-03-07
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  102     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-16     Completed Date:  2005-05-09     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4022-6     Citation Subset:  IM    
Affiliation:
Department of Physiology and Cellular Biophysics, Russ Berrie Medical Science Pavilion, Columbia University, 1150 St. Nicholas Avenue, New York, NY 10032, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brefeldin A / pharmacology
Endoplasmic Reticulum / physiology*
Golgi Apparatus / drug effects
Macrophages / physiology*
Membrane Proteins / physiology
Mice
Phagocytosis / drug effects,  physiology*
Protein Synthesis Inhibitors / pharmacology
R-SNARE Proteins
Vesicular Transport Proteins / physiology
Chemical
Reg. No./Substance:
0/ERS-24 protein, Cricetulus griseus; 0/Membrane Proteins; 0/Protein Synthesis Inhibitors; 0/R-SNARE Proteins; 0/Sec22b protein, mouse; 0/Vesicular Transport Proteins; 20350-15-6/Brefeldin A
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