| Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation. | |
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MedLine Citation:
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PMID: 19938108 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Histopathological evaluation of the liver via biopsy remains the standard procedure for the diagnosis of both acute cellular rejection (ACR) and recurrent hepatitis C (RHC) after liver transplantation. Nevertheless, it is often difficult to diagnose ACR in hepatitis C virus-positive recipients because of changes in common and overlapping with RHC. The aim of this study was to identify potential target genes for ACR in recipients with RHC. We analyzed 22 liver biopsy samples obtained from 21 hepatitis C virus-positive recipients. The clinicopathological diagnosis based on biopsy examination was ACR-predominant with superimposed RHC in 9 samples (ACR group) and RHC without ACR (non-ACR group) in 13. Using oligonucleotide microarrays, we compared the transcriptional changes in the 2 groups and selected 2206 genes that were significantly modulated in ACR. We analyzed the regulatory networks in ACR with Ingenuity Pathway Analysis software, and we confirmed with quantitative real-time polymerase chain reaction the reproducibility of caspase 8, apoptosis-related cysteine peptidase and bone morphogenetic protein 2 up-regulation in another group of validation samples, representing 2 genes from the core network as the target genes for ACR. Our results demonstrated novel transcriptome patterns for ACR with concurrent RHC that were distinct from those of recipients with only RHC, suggesting that gene expression profiling may be useful in the diagnosis of ACR in recipients with hepatitis C. |
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Authors:
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Tadafumi Asaoka; Tomoaki Kato; Shigeru Marubashi; Keizo Dono; Naoki Hama; Hidenori Takahashi; Shogo Kobayashi; Yutaka Takeda; Ichiro Takemasa; Hiroaki Nagano; Hideo Yoshida; Phillip Ruiz; Andreas G Tzakis; Kenichi Matsubara; Morito Monden; Yuichiro Doki; Masaki Mori |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society Volume: 15 ISSN: 1527-6473 ISO Abbreviation: Liver Transpl. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-30 Completed Date: 2010-01-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100909185 Medline TA: Liver Transpl Country: United States |
Other Details:
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Languages: eng Pagination: 1738-49 Citation Subset: IM |
Affiliation:
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Department of Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Adult Aged Biopsy Bone Morphogenetic Protein 2 / genetics Caspase 8 / genetics Female Gene Expression Profiling / methods* Gene Expression Regulation Gene Regulatory Networks Genetic Testing* Graft Rejection / diagnosis, genetics*, immunology, virology Hepatitis C / complications, diagnosis, genetics, surgery* Humans Liver Transplantation / adverse effects* Male Middle Aged NFATC Transcription Factors / genetics Oligonucleotide Array Sequence Analysis* Predictive Value of Tests Receptor, Interferon alpha-beta / genetics Receptors, Interleukin-12 / genetics Recurrence Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Treatment Outcome |
| Chemical | |
Reg. No./Substance:
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0/BMP2 protein, human; 0/Bone Morphogenetic Protein 2; 0/IFNAR1 protein, human; 0/IL12RB2 protein, human; 0/NFATC Transcription Factors; 0/NFATC3 protein, human; 0/Receptors, Interleukin-12; 156986-95-7/Receptor, Interferon alpha-beta; EC 3.4.22.-/CASP8 protein, human; EC 3.4.22.-/Caspase 8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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