| Differential susceptibility to carbapenems due to the AdeABC efflux pump among nosocomial outbreak isolates of Acinetobacter baumannii in a Chinese hospital. | |
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MedLine Citation:
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PMID: 18687557 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A nosocomial outbreak of carbapenem-resistant Acinetobacter baumannii occurred from February to November 2004 in the surgical intensive care unit (SICU) and the pediatric intensive care unit (PICU) of our hospital. Two separate clones prevailed, the antimicrobial susceptibility profiles of which were different. The PICU isolates produced OXA-23 oxacillinase, whereas no carbapenemases were detected from SICU isolates. No obvious outer membrane protein change was seen. Efflux pump phenotype was detected from SICU isolates. Efflux pump-encoding gene adeB was positive in these isolates and negative in PICU isolates. Through further study, we found that AdeABC efflux system gene contents were common in the SICU isolates. Then we compared the expression level of adeB in resistant and susceptible isolates using quantitative real-time polymerase chain reaction and found that increased expression of AdeABC efflux pump may play an important role in reduced meropenem susceptibility among A. baumannii in the SICU of our hospital. |
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Authors:
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Lei Huang; Liying Sun; Guobing Xu; Tiean Xia |
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Publication Detail:
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Type: Journal Article Date: 2008-08-08 |
Journal Detail:
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Title: Diagnostic microbiology and infectious disease Volume: 62 ISSN: 0732-8893 ISO Abbreviation: Diagn. Microbiol. Infect. Dis. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-24 Completed Date: 2009-04-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8305899 Medline TA: Diagn Microbiol Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 326-32 Citation Subset: IM |
Affiliation:
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Department of Clinical Laboratory, Peking University First Hospital, Beijing 100034, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter Infections
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drug therapy,
epidemiology,
microbiology* Acinetobacter baumannii / drug effects*, genetics, isolation & purification Adult Anti-Bacterial Agents / pharmacology* Bacterial Outer Membrane Proteins / genetics, metabolism Bacterial Proteins / genetics, metabolism* Carbapenems / pharmacology* Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology Child China / epidemiology Cross Infection / epidemiology, genetics, microbiology* Disease Outbreaks* Genes, Bacterial Humans Intensive Care Units Isoelectric Focusing Membrane Transport Proteins / genetics, metabolism* Microbial Sensitivity Tests Polymerase Chain Reaction beta-Lactam Resistance beta-Lactamases / genetics |
| Chemical | |
Reg. No./Substance:
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0/AdeA protein, Acinetobacter baumannii; 0/AdeB protein, Acinetobacter baumannii; 0/AdeC protein, Acinetobacter baumannii; 0/Anti-Bacterial Agents; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Carbapenems; 0/Membrane Transport Proteins; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; EC 3.5.2.6/beta-Lactamases; EC 3.5.2.6/carbapenemase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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