Document Detail

Differential susceptibility of different cell lines to swine-origin influenza A H1N1, seasonal human influenza A H1N1, and avian influenza A H5N1 viruses.
MedLine Citation:
PMID:  19801200     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The novel swine-origin influenza A H1N1 virus (S-OIV) causes the current pandemic. Its tissue tropism and replication in different cell lines are not well understood. OBJECTIVE: Compare the growth characteristics of cell lines infected by S-OIV, seasonal influenza A H1N1 (sH1N1) and avian influenza A H5N1 (H5N1) viruses and the effect of temperature on viral replication. STUDY DESIGN: Cytopathic effect (CPE), antigen expression by immunofluorescence (IF) and viral load profile by quantitative RT-PCR in 17 cell lines infected by S-OIV, sH1N1 and H5N1 were examined. Comparison of their replication efficiency in chick embryo was performed. The effect of temperature on viral replication in Madin-Darby canine kidney (MDCK) cells was determined by TCID(50) at 33 degrees C, 37 degrees C and 39 degrees C for 5 consecutive days. RESULTS: S-OIV replicated in cell lines derived from different tissues or organs and host species with comparable viral load to sH1N1. Among 13 human cell lines tested, Caco-2 has the highest viral load for S-OIV. S-OIV showed a low viral load with no CPE or antigen expression in pig kidney cell PK-15, H5N1 demonstrated the most diverse cell tropism by CPE and antigen expression, and the highest viral replication efficiency in both cell lines and allantoic fluid. All three viruses demonstrated best growth at 37 degrees C in MDCK cells. CONCLUSION: Cell line growth characteristics of S-OIV, sH1N1 and H5N1 appear to correlate clinically and pathologically with involved anatomical sites and severity. Low replication of S-OIV in PK-15 suggests that this virus is more adapted to human than swine.
I W S Li; K H Chan; K W K To; S S Y Wong; P L Ho; S K P Lau; P C Y Woo; H W Tsoi; J F W Chan; V C C Cheng; B J Zheng; H Chen; K Y Yuen
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-03
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  46     ISSN:  1873-5967     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-09     Completed Date:  2010-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  325-30     Citation Subset:  IM    
Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
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MeSH Terms
Birds / virology
Cell Line
Cell Line, Tumor
Chick Embryo
Influenza A Virus, H1N1 Subtype / physiology*
Influenza A Virus, H5N1 Subtype / physiology*
Influenza in Birds / virology*
Influenza, Human / virology*
Swine / virology
Swine Diseases / virology*
Viral Load
Virus Replication*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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