Document Detail


Differential surface marker expression in patients with CD-16+ lymphoproliferative disorders: in vivo model for NK differentiation.
MedLine Citation:
PMID:  3475265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we report on three patients, each with a CD-16+ lymphoproliferative disorder. Peripheral blood lymphocyte from all three patients were evaluated for lymphocyte morphology, natural killer (NK) function, and surface marker expression. In addition, two-color flow cytometric analysis was performed to determine the phenotype of the CD-16+ cells. Our findings indicate that the presence of increased numbers of CD-16+ cells alone is not a good predictor of NK activity. However, we observed a differential expression of the HLA class II molecules DR and DQ on the CD-16+ cells obtained from these patients that was associated with NK function. Hence, a CD-16+, Leu-7-, Leu-19+ (NKH-1A) and HLA class II+ phenotype did correlate with NK function in contrast to a CD-16+, Leu-7+, Leu-19- (NKH-1A) and HLA class II- phenotype. Of importance was the fact that the CD-16+, HLA class II+ cells did not express CD-25 or TFR, nor did they mediate cytotoxicity against solid tumor targets, suggesting that these CD-16+ cells are not activated. Thus, in contrast to previous studies of NK ontogeny that utilized in vitro activated NK cells, studies of patients with CD-16+ lymphoproliferative disorders may provide an alternative approach for examining NK differentiation.
Authors:
R A Bray; L R Gottschalk; A L Landay; H M Gebel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human immunology     Volume:  19     ISSN:  0198-8859     ISO Abbreviation:  Hum. Immunol.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-08-28     Completed Date:  1987-08-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8010936     Medline TA:  Hum Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  105-15     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Monoclonal
Antigens, Surface / analysis*
Cell Differentiation
Female
Flow Cytometry
HLA-DQ Antigens / analysis
HLA-DR Antigens / analysis
Humans
Killer Cells, Natural / immunology
Leukemia, Lymphoid / immunology*
Leukemia, Myeloid / immunology*
Lymphocytes / immunology*
Male
Middle Aged
Phenotype
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Surface; 0/HLA-DQ Antigens; 0/HLA-DR Antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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