Document Detail


Differential staining of the X-chromosome during meiosis of orthoptera by a silver impregnation procedure.
MedLine Citation:
PMID:  6181945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differential staining of the X chromosome of Orthoptera, which normally cannot be distinguished from the autosomes during the more condensed stages of meiosis, has been achieved by a silver impregnation procedure involving a pretreatment with 2 x SSC at 60 degrees C. The results suggest that the saline citrate solution preferentially extracts proteins from the X chromosome. This method may be useful for distinguishing chromosome regions composed of facultative heterochromatin in Orthoptera.
Authors:
J Fernandez-Piqueras; A R Campos; C S Castaño; E R Garcia
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chromosoma     Volume:  85     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1982  
Date Detail:
Created Date:  1982-12-18     Completed Date:  1982-12-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  707-11     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Heterochromatin / ultrastructure
Male
Meiosis
Orthoptera / ultrastructure*
Sex Chromosomes / ultrastructure*
Staining and Labeling / methods*
X Chromosome / ultrastructure*
Chemical
Reg. No./Substance:
0/Heterochromatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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