Document Detail

Differential staining of Chironomus thummi giant chromosomes by treatment with acridine orange after mild acid hydrolysis.
MedLine Citation:
PMID:  74309     Owner:  NLM     Status:  MEDLINE    
The fluorescence of Chironomus thummi giant chromosomes stained by acridine orange after mild acid hydrolysis (1N HCl at 37 degrees) has been studied. After very short hydrolysis as well as in control preparations (untreated by HCl) all chromosome regions show green fluorescence. After long hydrolysis (10 min) all chromosome bands fluoresce red. Intermediate time of hydrolysis (2 min) give in all transcriptionally inactive bands including centromeric ones red fluorescence whereas transcriptionally active puffing regions are green. The possible mechanisms of differential staining of individual chromosome regions are discussed. It is suggested that transcriptionally active chromosome regions are less susceptible to the action of acid or that the difference in chromatin stainability after acid-AO treatment is due to the difference in chromatin packing. The applicability of this method to the study of functional states of chromatin, at least in giant chromosomes, is discussed.
A V Zelenin; N G Stepanova; I I Kiknadze
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chromosoma     Volume:  64     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-03-10     Completed Date:  1978-03-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  327-35     Citation Subset:  IM    
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MeSH Terms
Chromosomes / metabolism,  ultrastructure*
Diptera / ultrastructure*
Hydrochloric Acid
Microscopy, Fluorescence
RNA / metabolism
Staining and Labeling
Reg. No./Substance:
0/Acridines; 63231-63-0/RNA; 7647-01-0/Hydrochloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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