| Differential roles of Annexin A1 (ANXA1/lipocortin-1/lipomodulin) and thioredoxin binding protein-2 (TBP-2/VDUP1/TXNIP) in glucocorticoid signaling of HTLV-I-transformed T cells. | |
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MedLine Citation:
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PMID: 20398702 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucocorticoid (GC) is widely used for therapeutic purposes in immunological and hematological disorders. Annexin A1 (ANXA1/lipocortin-1/lipomodulin), a GC-inducible molecule, was regarded as a vital anti-inflammatory mediator of GC. Thioredoxin binding protein-2 (TBP-2/VDUP1/TXNIP), a regulator of redox reactions, cell growth and lipid metabolism, was also reportedly induced by GC. HTLV-I infected T cells undergo the transition from the IL-2 dependent to IL-2 independent growth during the long-term culture in vitro. We found that these T cells responded to GC with growth arrest and apoptosis in the IL-2 dependent growth stage, whereas they failed to respond to GC after their growth had shifted into the IL-2 independent stage. Here we employed these T cell lines and studied the roles of ANXA1 and TBP-2 in mediating GC-induced apoptosis. In GC-sensitive T cells, ANXA1 expression was negligible and unaffected by GC treatment, whereas TBP-2 was expressed and induced by GC treatment. In GC-resistant T cells, however, ANXA1 was highly expressed regardless of GC treatment and promoted cellular proliferation. In contrast, TBP-2 expression was lost and could not mediate the GC-induced apoptosis. In conclusion, these results suggest that TBP-2, but not ANXA1, is directly involved in the switching of GC sensitivity and GC resistance in HTLV-I infected T cell lines, whereas ANXA1 may be a biomarker indicative of the advanced stage of the transformation. |
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Authors:
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Zhe Chen; Eiji Yoshihara; Aoi Son; Yoshiyuki Matsuo; Hiroshi Masutani; Katsuji Sugie; Michiyuki Maeda; Junji Yodoi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-14 |
Journal Detail:
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Title: Immunology letters Volume: 131 ISSN: 1879-0542 ISO Abbreviation: Immunol. Lett. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-08-04 Completed Date: 2010-12-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7910006 Medline TA: Immunol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 11-8 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Laboratory of Infection and Prevention, Department of Biological Responses, Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Annexin A1
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genetics,
metabolism* Apoptosis Carrier Proteins / genetics, metabolism* Cell Line, Transformed Cell Proliferation Cell Transformation, Viral* Gene Expression Regulation Glucocorticoids / metabolism, pharmacology* Human T-lymphotropic virus 1 / physiology* Humans Interleukin-2 / metabolism Leukemia-Lymphoma, Adult T-Cell Lymphocyte Activation Signal Transduction / drug effects* T-Lymphocytes / metabolism, physiology, virology* |
| Chemical | |
Reg. No./Substance:
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0/Annexin A1; 0/Carrier Proteins; 0/Glucocorticoids; 0/Interleukin-2; 0/TXNIP protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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