| Differential role of platelet granular mediators in angiogenesis. | |
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MedLine Citation:
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PMID: 15249180 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Platelets contain numerous substances regulating angiogenic response. However, the regulatory role of platelets in blood vessel development remains to be elucidated. We investigated the comprehensive effect of platelets as a cellular system on angiogenesis. METHODS: The following approaches were applied: (a) in vitro-aortic ring assay and chemotaxis assay; (b) in vivo-injection of platelet-containing matrigel plug subcutaneously into a mouse followed by immunohistochemical analysis of angiogenic response. RESULTS: Platelets stimulated formation of blood vessels in vitro in the rat aortic ring model via VEGF and bFGF, while blocking of platelet factor-4 promoted this effect. Addition of platelets to the matrigel followed by its subcutaneous injection into a mouse resulted in an intensive migration of fibroblasts into the matrigel as well as formation of blood capillaries de novo. This platelet effect was mediated through bFGF, VEGF, and heparanase. Furthermore, platelet releasate was found to induce endothelial cell chemotaxis. This effect was mediated by a concerted action of intraplatelet bFGF, PDGF, VEGF, and heparanase. CONCLUSION: Platelets affect different stages of the angiogenic response with a trend to a pro-angiogenic net effect despite the presence of angiogenesis inhibitors such as platelet factor 4. While a concomitant effect of bFGF and VEGF seemed to be essential for the entire process of vessel formation (aortic ring and matrigel models), PDGF and heparanase were effective only at the migration stage. |
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Authors:
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Alexander Brill; Hila Elinav; David Varon |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cardiovascular research Volume: 63 ISSN: 0008-6363 ISO Abbreviation: Cardiovasc. Res. Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-07-13 Completed Date: 2004-10-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 226-35 Citation Subset: IM |
Copyright Information:
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Copyright 2004 European Society of Cardiology |
Affiliation:
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Blood Coagulation Unit, Hematology Department, Hadassah Medical Center, Jerusalem, Israel. brilla@hadassah.org.il |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Blocking / pharmacology Aorta Blood Platelets / physiology* Capillaries / pathology Cell Movement Collagen Drug Combinations Endothelial Cells / metabolism, pathology Endothelium, Vascular / metabolism, pathology* Fibroblast Growth Factor 2 / immunology, metabolism Fibroblasts / pathology Heparin Lyase / antagonists & inhibitors, metabolism Humans Laminin Male Mice Mice, Inbred Strains Neovascularization, Pathologic* Platelet Factor 4 / immunology, metabolism Platelet-Derived Growth Factor / immunology, metabolism Proteoglycans Rats Rats, Inbred Strains Vascular Endothelial Growth Factor A / antagonists & inhibitors, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Blocking; 0/Drug Combinations; 0/Laminin; 0/Platelet-Derived Growth Factor; 0/Proteoglycans; 0/Vascular Endothelial Growth Factor A; 103107-01-3/Fibroblast Growth Factor 2; 119978-18-6/matrigel; 37270-94-3/Platelet Factor 4; 9007-34-5/Collagen; EC 4.2.2.7/Heparin Lyase |
| Comments/Corrections | |
Comment In:
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Cardiovasc Res. 2004 Aug 1;63(2):192-3
[PMID:
15249175
]
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Erratum In:
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Cardiovasc Res. 2004 Nov 1;64(2):374 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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