| Differential responsiveness of proximal tubule segments to metabolic inhibitors in the isolated perfused rat kidney. | |
| | |
MedLine Citation:
|
PMID: 3942135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Rat kidneys were perfused for 90 minutes with cyanide, rotenone, antimycin, 2-deoxyglucose, or combinations of rotenone or antimycin with 2-deoxyglucose in oxygenated Krebs-albumin medium. Following perfusion, proximal tubule injury was evaluated by light microscopy. The types of lesions seen were similar to those previously reported after hypoxic perfusion and included brush border clubbing/mitochondrial swelling in S1 and S2 and cytoplasmic edema or cell fragmentation in S3. This finding supports the contention that these lesions represent characteristic responses of these segment types and that the S3 response differs from that in S1 and S2. S1 appeared most vulnerable to low dose cyanide or inhibition of mitochondrial electron transport (rotenone, antimycin). Inhibition of glycolysis (2-deoxyglucose) only produced injury in S2 tubules. With high dose cyanide or a combination of 2-deoxyglucose with either rotenone or antimycin, there was diffuse proximal tubule damage. Thus S3 appeared more resistant than the convoluted tubular segments to both inhibition of glycolysis and to inhibition of mitochondrial electron transport. This finding stands in contrast to the selective vulnerability of the S3 segment in ischemic renal injury. |
| | |
Authors:
|
P F Shanley; M Brezis; K Spokes; P Silva; F H Epstein; S Rosen |
Related Documents
:
|
535885 - Evidence for na-retaining humoral agents and vasoconstrictor humoral agents in hyperten... 3355595 - Synergistic effects of hypoxia and fasting on harmol elimination in the isolated perfus... 6295185 - Anion effects on fluid absorption from rat jejunum perfused in vivo. 1984365 - Amino acids fail to prevent halothane depression of albumin synthesis: studies in the i... 2034035 - Effect of aging on phosphate metabolites of rat brain as revealed by the in vivo and in... 3665855 - Mesovarian leiomyomas in the rat. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: American journal of kidney diseases : the official journal of the National Kidney Foundation Volume: 7 ISSN: 0272-6386 ISO Abbreviation: Am. J. Kidney Dis. Publication Date: 1986 Jan |
Date Detail:
|
Created Date: 1986-02-17 Completed Date: 1986-02-17 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8110075 Medline TA: Am J Kidney Dis Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 76-83 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antimycin A / analogs & derivatives*, pharmacology Biological Transport, Active / drug effects Cyanides / pharmacology* Deoxy Sugars / pharmacology* Deoxyglucose / pharmacology* Glycolysis / drug effects Kidney Kidney Tubules, Proximal / drug effects*, ultrastructure Male Microvilli / drug effects Mitochondrial Swelling / drug effects Perfusion Potassium Cyanide / pharmacology* Rats Rats, Inbred Strains Rotenone / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
|
AM 18078/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cyanides; 0/Deoxy Sugars; 11118-72-2/antimycin; 151-50-8/Potassium Cyanide; 154-17-6/Deoxyglucose; 642-15-9/Antimycin A; 83-79-4/Rotenone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: An ultrastructural study of the renal juxtaglomerular apparatus and extraglomerular mesangium in pat...
Next Document: Morphologic diagnosis of acute tubular necrosis (ATN) by autofluorescence.