| Differential responses of proliferating versus quiescent cells to adriamycin. | |
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MedLine Citation:
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PMID: 10388527 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The relative sensitivity of proliferating and quiescent cells to DNA-damaging agents is a key factor for cancer chemotherapy. Here we undertook a reevaluation of the way that proliferating and quiescent cells differ in their responses and fate to adriamycin-induced damage. Distinct types of assays that measure membrane integrity, metabolic activity, cell size, DNA content, and the ability to proliferate were used to compare growing and quiescent Swiss3T3 fibroblasts after adriamycin treatment. We found that immediately after adriamycin treatment of growing cells, p53 and p21(Cip1/Waf1) were induced but the cells remained viable. In contrast, less p53 and p21(Cip1/Waf1) were induced in quiescent cells after adriamycin treatment, but the cells were more prone to immediate cell death, possibly involving apoptosis. Adriamycin induced a G2/M cell cycle arrest in growing cells and a concomitant increase in cell size. In contrast, adriamycin induced an increase in sub-G1 DNA content in quiescent cells and a decrease in cell size. In contrast to the short-term responses, adriamycin-treated quiescent cells have a better long-term survival and proliferation potential than adriamycin-treated growing cells in colony formation assays. These data suggest that proliferating and resting cells are remarkably different in their short-term and long-term responses to adriamycin. |
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Authors:
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W Y Siu; T Arooz; R Y Poon |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 250 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1999 Jul |
Date Detail:
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Created Date: 1999-08-25 Completed Date: 1999-08-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 131-41 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Antibiotics, Antineoplastic / pharmacology* Cell Cycle Cell Division / drug effects Cell Survival / drug effects Cyclin-Dependent Kinase Inhibitor p21 Cyclins / biosynthesis Doxorubicin / pharmacology* Mice Rats Trypan Blue Tumor Suppressor Protein p53 / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Cdkn1a protein, mouse; 0/Cdkn1a protein, rat; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Tumor Suppressor Protein p53; 23214-92-8/Doxorubicin; 72-57-1/Trypan Blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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