Document Detail


Differential responses from seven mammalian cell lines to the treatments of detoxifying enzyme inducers.
MedLine Citation:
PMID:  12628444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-based models have been used extensively in screening novel bioactive chemical entities. In this study, seven well-established mammalian cell lines, which have different origins, were utilized to compare their responses to the treatments of three detoxifying enzyme inducers, tert-butylhydroquinone (tBHQ), beta-naphthoflavone (beta-NF), and sulforaphane (SUL), which are potential chemopreventive compounds. The enzymatic activities of glutathione s-transferase (GST), NAD(P)H:quinone oxidoreductase (QR), aldehyde reductase (AR), and glutathione reductase (GR) were measured by kinetics methods using UV-Vis spectroscopy, and analyzed statistically by Student's t-test. Among these mammalian cell lines, the mouse hepatoma Hepa1c1c7 cells were the most robust and sensitive cells, which had higher basal as well as upregulated enzymatic activities. In human cell lines, the prostate LNCaP and hepatic HepG2 cells were also very responsive to the inducers. The results suggested that different cell lines responded differently to individual detoxifying gene inducer, and the selection of appropriate cell line is important for screening potential chemopreventive agents.
Authors:
Zhi-Qiang Jiang; Chi Chen; Bo Yang; Vidya Hebbar; A-N Tony Kong
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Life sciences     Volume:  72     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-11     Completed Date:  2003-04-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2243-53     Citation Subset:  IM    
Affiliation:
Center for Pharmaceutical Biotechnology, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60607, USA.
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MeSH Terms
Descriptor/Qualifier:
Aldehyde Reductase / biosynthesis
Animals
Anticarcinogenic Agents / pharmacology
Chemoprevention
Drug Screening Assays, Antitumor
Enzyme Induction*
Enzyme Inhibitors / pharmacology
Female
Glutathione Reductase / biosynthesis
Glutathione Transferase / biosynthesis*
Humans
Hydroquinones / pharmacology
Male
Metabolic Detoxication, Drug* / physiology
Mice
NAD(P)H Dehydrogenase (Quinone) / biosynthesis
Oxidoreductases / biosynthesis*
Thiocyanates / pharmacology
Tumor Cells, Cultured / drug effects,  enzymology*
beta-Naphthoflavone / pharmacology
Grant Support
ID/Acronym/Agency:
R01-CA73674/CA/NCI NIH HHS; R01-CA94828/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Anticarcinogenic Agents; 0/Enzyme Inhibitors; 0/Hydroquinones; 0/Thiocyanates; 1948-33-0/2-tert-butylhydroquinone; 4478-93-7/sulforafan; 6051-87-2/beta-Naphthoflavone; EC 1.-/Oxidoreductases; EC 1.1.1.21/Aldehyde Reductase; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone); EC 1.8.1.7/Glutathione Reductase; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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