Document Detail


Differential response of normal human epidermal keratinocytes and HaCaT cells to hydrogen peroxide-induced oxidative stress.
MedLine Citation:
PMID:  22439662     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background.  Normal human epidermal keratinocytes (NHEKs) and HaCaT cells are the most common models used to study the effects of various factors on skin cells. These cell lines share some common characteristics, but little is known about their differences in handling hydrogen peroxide (H(2) O(2) )-induced oxidative stress. Aim.  To investigate the differential response of NHEKs and HaCaT cells to H(2) O(2) -induced oxidative stress. Methods.  We examined differences in NHEKs and HaCaT cells after H(2) O(2) treatment, assessing changes in cell viability; levels of intracellular reactive oxygen species (ROS), superoxide dismutase (SOD) and caspase-3/7; percentage of cells arrested in G1 phase; number of senescence-associated β-galactosidase (SA-β-Gal)-positive cells and; expression of senescence-related protein Klotho. Results.  The viability of NHEKs and HaCaT cells decreased in a concentration-dependent and time-dependent manner after exposure to H(2) O(2) . The inhibitory effect of 150 μmol/L H(2) O(2) on cell viability was greater in HaCaT cells than in NHEKs (P < 0.05). Levels of ROS and caspase-3/7, and the percentage of cells arrested in G1 phase, were higher in HaCaT cells than in NHEKs, whereas intracellular SOD was higher in NHEKs than in HaCaT cells after exposure to 150 μmol/L H(2) O(2) (P < 0.05). SA-β-Gal positive cells increased significantly in NHEKs after treatment with H(2) O(2) (P < 0.05). Klotho was significantly downregulated in both NHEKs and HaCaT cells after H(2) O(2) treatment, but no SA-β-Gal-positive HaCaT cells were seen, even after treatment with H(2) O(2) . Conclusions.  Normal human epidermal keratinocytes are more resistant than HaCaT cells to H(2) O(2) -induced oxidative stress. HaCaT cells have senescence phenotypes, but do not express β-Gal.
Authors:
L Liu; H Xie; X Chen; W Shi; X Xiao; D Lei; J Li
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-22
Journal Detail:
Title:  Clinical and experimental dermatology     Volume:  -     ISSN:  1365-2230     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7606847     Medline TA:  Clin Exp Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© The Author(s). CED © 2012 British Association of Dermatologists.
Affiliation:
Department of Dermatology, Xiang Ya Hospital, Central South University, Hunan, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evaluation of WO2012003387, Gilead's ASK1 inhibitors.
Next Document:  Graphene Nano Platelet Induced Strengthening of Ultra High Molecular Weight Polyethylene and Biocomp...