Document Detail


Differential response of mesoderm- and neural crest-derived smooth muscle to TGF-beta1: regulation of c-myb and alpha1 (I) procollagen genes.
MedLine Citation:
PMID:  9024776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previously, we demonstrated that avian vascular smooth muscle cells (VSMC) derived from embryonic abdominal and thoracic aorta grow differently in the presence of transforming growth factor beta (TGF-beta1) and platelet-derived growth factor (PDGF-BB) (Wrenn et al., In Vitro Cell. Dev. Biol. 29, 73-78, 1992). The thoracic VSMC (N-VSMC) are derived from neural crest, and therefore differentiate from ectoderm; the abdominal VSMC (M-VSMC) are derived from mesoderm. The present study was designed to identify factors that mediate the differential responses of the VSMC to TGF-beta1. We found that TGF-beta1 increased DNA synthesis by approximately sevenfold in N-VSMC. Levels of both alpha1 (I) procollagen and c-myb mRNAs were markedly induced in N-VSMC treated with TGF-beta1. Chimeric plasmids containing up to 3.5 kb of alpha1 (I) procollagen 5' flanking DNA were induced to equivalent levels as procollagen mRNA in N-VSMC. However, TGF-beta1 increased DNA synthesis by threefold in M-VSMC; there was no effect on alpha1 (I) procollagen expression, and c-myb was not expressed, as demonstrated by immunohistochemistry staining and RNA analyses. Antisense c-myb oligodeoxynucleotides blocked the TGF-beta1 induction of alpha1 (I) procollagen and the growth of N-VSMC. The increase in DNA synthesis by M- and N-VSMC was correlated with the secretion of PDGF-AA, and staurosporine and antibodies directed against PDGF-AA suppressed DNA synthesis. Our results demonstrate that TGF-beta1 activity and c-myb expression modulate the expression of alpha1 (I) collagen and cell proliferation in neural crest-derived smooth muscle. The regulation of these events by TGF-beta1 may be important during morphogenesis of blood vessels and vascular diseases.
Authors:
P F Gadson; M L Dalton; E Patterson; D D Svoboda; L Hutchinson; D Schram; T H Rosenquist
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  230     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-14     Completed Date:  1997-03-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  169-80     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha 68195-6395, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta, Abdominal / cytology,  drug effects*,  embryology,  metabolism
Aorta, Thoracic / cytology,  drug effects*,  embryology,  metabolism
Birds / embryology
Cells, Cultured
Coronary Vessels / cytology,  drug effects*,  embryology,  metabolism
Gene Expression Regulation / drug effects*
Mesoderm
Muscle, Smooth, Vascular / cytology,  drug effects*,  embryology,  metabolism
Platelet-Derived Growth Factor / metabolism
Procollagen / genetics*
Proto-Oncogene Proteins c-myc / genetics*,  metabolism
RNA, Messenger
Thymidine / pharmacokinetics
Transforming Growth Factor beta / pharmacology*
Grant Support
ID/Acronym/Agency:
HL-42164-06/HL/NHLBI NIH HHS; HL-45337-05/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Platelet-Derived Growth Factor; 0/Procollagen; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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