Document Detail

Differential response of adenylate cyclase and ATPase activities after chronic ethanol exposure of PC12 cells.
MedLine Citation:
PMID:  2843606     Owner:  NLM     Status:  MEDLINE    
The direct effects of chronic ethanol administration on adenylate cyclase, Na,K-ATPase, and Mg-ATPase activities in a cell containing neuronal characteristics were investigated using PC12 pheochromocytoma cells. Exposure of PC12 cells to 0, 75, and 150 mM ethanol for 4 days caused a dose-dependent increase in the stimulation of adenylate cyclase by in vitro ethanol without altering activation of the enzyme by GTP, NaF, MnCl2, or 2-chloroadenosine. Conversely, a 4-day treatment with 150 mM ethanol increased Na,K-ATPase and Mg-ATPase activities without altering the inhibitory effects of in vitro ethanol. The increase in Na,K-ATPase activity was associated with an increase in Vmax without any change in the Km for KCl. Chronic ethanol exposure also increased the amount of [3H]ouabain specifically bound to PC12 cell membranes. Except for the increase in Mg-ATPase activity, the above results were also observed when chronic ethanol treatment was carried out in the presence of pyrazole. Although ethanol slowed PC12 cell growth, observed changes were not due to an ethanol-induced reduction in cellular density. A 4-day exposure of a nonneuronal cell line (Madin Darby canine kidney cell) to 150 mM ethanol did not alter adenylate cyclase or ATPase activities. The present study indicates that the direct effects of chronic ethanol exposure of a neuronal-like cell involve an increase in the density of sodium pumps per cell and an enhanced sensitivity of adenylate cyclase to activation by ethanol.
R A Rabin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  51     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-10-19     Completed Date:  1988-10-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1148-55     Citation Subset:  IM    
Department of Pharmacology and Therapeutics, SUNY-Buffalo 14214.
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MeSH Terms
Adenosine / analogs & derivatives,  pharmacology
Adenylate Cyclase / metabolism*
Adrenal Gland Neoplasms / enzymology*
Ca(2+) Mg(2+)-ATPase / metabolism*
Enzyme Activation / drug effects
Ethanol / pharmacology*
Guanosine Triphosphate / pharmacology
Guanylyl Imidodiphosphate / pharmacology
Manganese / pharmacology
Manganese Compounds*
Neurons / drug effects,  enzymology
Ouabain / metabolism
Pheochromocytoma / enzymology*
Sodium Fluoride / pharmacology
Sodium-Potassium-Exchanging ATPase / metabolism*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Chlorides; 0/Manganese Compounds; 146-77-0/2-Chloroadenosine; 34273-04-6/Guanylyl Imidodiphosphate; 58-61-7/Adenosine; 630-60-4/Ouabain; 64-17-5/Ethanol; 7439-96-5/Manganese; 7681-49-4/Sodium Fluoride; 7773-01-5/manganese chloride; 86-01-1/Guanosine Triphosphate; EC 3.6.1.-/Ca(2+) Mg(2+)-ATPase; EC ATPase; EC Cyclase

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