| Differential repression by the transcription factor REST/NRSF of the various Ca2+ signalling mechanisms in pheochromocytoma PC12 cells. | |
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MedLine Citation:
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PMID: 20171735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Expression of the nerve cell phenotype is orchestrated by the REST/NRSF transcription repressor, working on hundreds of genes recognized at a specific regulatory binding sequence. Most PC12 clones, the most frequently employed neuronal model, maintain low levels of REST; however a few, defective of neurosecretion, express high levels. To investigate the role of REST in Ca2+ signalling we studied the [Ca2+](i) changes in single cells of four clones, two wild-type and two defective, pre-treated for 5 days with NGF. We focused on Ca2+ influxes induced by depolarization and ATP. Only a subpopulation ( approximately 15%) of the defective, high REST cells responded to depolarization (Ca(V) expression approximately 10%). The ATP-induced intracellular Ca2+ release was little changed, whereas influx via ionotropic P2X receptors was decreased, in agreement with the decreased expression of P2X2 receptors. The percentage of defective cells expressing store-operated calcium entry (SOCE) following ATP stimulation was also lower. The responses of the defective clones were little affected by their differentiated state. In conclusion, our results revealed important new aspects of REST control of Ca2+ homeostasis, of potential physiological importance. The mechanisms of this control remain to be investigated. |
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Authors:
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P Ariano; P Zamburlin; R D'Alessandro; J Meldolesi; D Lovisolo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-19 |
Journal Detail:
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Title: Cell calcium Volume: 47 ISSN: 1532-1991 ISO Abbreviation: Cell Calcium Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-05 Completed Date: 2010-09-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006226 Medline TA: Cell Calcium Country: Netherlands |
Other Details:
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Languages: eng Pagination: 360-8 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Animal and Human Biology, University of Turin, via Accademia Albertina 13, I-10123 and NIS Centre of Excellence, Turin, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Calcium Signaling / physiology Cell Differentiation Enzyme Repression Membrane Potentials Nerve Growth Factor / metabolism Neurons / physiology* Neurosecretion / genetics PC12 Cells Pheochromocytoma / genetics, metabolism*, pathology Rats Receptors, Purinergic P2 / genetics, metabolism* Repressor Proteins / genetics, metabolism* TRPC Cation Channels / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/RE1-silencing transcription factor; 0/Receptors, Purinergic P2; 0/Repressor Proteins; 0/TRPC Cation Channels; 0/purinergic P2X receptor; 56-65-5/Adenosine Triphosphate; 9061-61-4/Nerve Growth Factor |
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