Document Detail


Differential release of membrane-bound alkaline phosphatase isoenzymes from tumor cells by bromelain.
MedLine Citation:
PMID:  7052949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid enzymatic method is presented which results in the selective release of cell-surface alkaline phosphatase isoenzymes. Treatment of suspensions of human tumor cell lines with the proteolytic enzyme bromelain released certain alkaline phosphatase isoenzymes into low-molecular-weight, catalytically active forms. Cells which expressed term placental or intestinal isoenzymes were equally susceptible to this treatment. A cell line which expressed early placental isoenzyme, however, was unaffected by bromelain as indicated by complete recovery of activity in the treated membrane fraction. Successful solubilization of immunologically reactive enzyme allows quantitation of levels of cell-surface enzyme in response to modulators of gene expression. Moreover, the observed selective solubilization of isoenzymes by bromelain may be of general use in analyses of the physical association between other biologically important surface proteins and the lipid components of the cell membrane.
Authors:
R H Kottel; W C Hanford
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  2     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  1980 Jun 
Date Detail:
Created Date:  1983-08-11     Completed Date:  1983-08-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  325-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / analysis*
Bromelains / pharmacology*
Cell Membrane / enzymology
Hela Cells
Humans
Immunoelectrophoresis
Isoenzymes / analysis
Solubility
Grant Support
ID/Acronym/Agency:
R01-21967//PHS HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; 9001-00-7/Bromelains; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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