Document Detail


Differential regulation of prostaglandin endoperoxide synthase-2 transcription in ovine granulosa and large luteal cells.
MedLine Citation:
PMID:  11377586     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objective of the present study was to evaluate second messenger regulation of prostaglandin synthase-2 (PGS-2) mRNA and PGS-2 promoter in ovine granulosa cells and large luteal cells. In granulosa cells, PGS-2 mRNA was induced by forskolin (PKA activator) but not by phorbol didecanoate (PDD; PKC activator) with maximal stimulation at 24 h. In contrast, PDD was the more potent inducer in large luteal cells with the most dramatic effect by 4 h. Similarly, forskolin but not PDD increased media PGF(2alpha) in granulosa cells at 24 h; whereas, PDD but not forskolin increased PGF(2alpha) at 4 h and 24 h in large luteal cells. To evaluate if these effects were due to activation of transcription, a plasmid was constructed containing 1500 bp of PGS-2 promoter linked to a luciferase gene. Forskolin stimulated transcription from this construct in granulosa cells (5-fold); whereas, PDD but not forskolin stimulated transcription in large luteal cells (40-fold). Taking these findings together, we propose that transcriptional regulation of the PGS-2 gene changes from PKA-dependence in granulosa cells to PKC-dependence in large luteal cells after luteinization.
Authors:
Y -L. Wu; M C. Wiltbank
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Prostaglandins     Volume:  65     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-May-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  -    
Other Details:
Languages:  ENG     Pagination:  103-116     Citation Subset:  -    
Affiliation:
Endocrinology-Reproductive Physiology Program, University of Wisconsin-Madison, Madison, Wisconsin, USA
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