Document Detail

Differential regulation of primary response gene expression in skeletal muscle cells through multiple signal transduction pathways.
MedLine Citation:
PMID:  7718627     Owner:  NLM     Status:  MEDLINE    
One of the earliest cellular responses to growth factors is the rapid induction of primary response genes. One group of such genes was originally isolated as tetradecanoyl phorbol acetate (TPA) inducible sequences (TIS genes) from mouse 3T3 cells. Proteins encoded by the TIS genes include two transcription factors: TIS8 (also known as egr1/NGFIA/zif268) and TIS1 (also known as NGFIB/nur77/N10). We have examined the inducibility of these two genes in a skeletal muscle cell line in response to agents that have been reported to block muscle differentiation. We report here that basic fibroblast growth factor (bFGF) induced the expression of both TIS1 and TIS8 in mouse C2C12cells. Both genes were also inducible by TPA while forskolin which activates the cAMP-dependent pathway induced TIS1 but not TIS8. Down-regulation of protein kinase C (PKC) activity by TPA pretreatment repressed the bFGF induction of TIS1 but had little effect on the bFGF-stimulated expression of TIS8. Moreover, while both TPA and bFGF stimulated the hyperphosphorylation of c-RAF and the activity of MAP kinase, TPA pretreatment failed to block RAF phosphorylation or the stimulation of MAP kinase activity by bFGF. Induction of the two TIS genes in skeletal myoblasts therefore appeared to be dependent to different extents on the activation of protein kinase A (PKA), PKC and MAP kinase.
R W Lim; C Y Zhu; B Stringer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1266     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-24     Completed Date:  1995-05-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  91-100     Citation Subset:  IM    
Department of Pharmacology, University of Missouri-Columbia 65212, USA.
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MeSH Terms
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
Cell Line
DNA-Binding Proteins / genetics*
Fibroblast Growth Factor 2 / pharmacology
Forskolin / pharmacology
Gene Expression Regulation / drug effects
Muscles / metabolism*
Nuclear Receptor Subfamily 4, Group A, Member 1
Protein Kinase C / metabolism
Receptors, Cytoplasmic and Nuclear
Receptors, Steroid
Signal Transduction / genetics*
Tetradecanoylphorbol Acetate / pharmacology
Transcription Factors / genetics*
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Nr4a1 protein, mouse; 0/Nuclear Receptor Subfamily 4, Group A, Member 1; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, Steroid; 0/Transcription Factors; 103107-01-3/Fibroblast Growth Factor 2; 16561-29-8/Tetradecanoylphorbol Acetate; 66428-89-5/Forskolin; EC Kinase C; EC Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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