Document Detail


Differential regulation of the major cyclin-dependent kinases, cdk2 and cdc2, during cell cycle progression in human lymphocytes exposed to heptachlor.
MedLine Citation:
PMID:  10757037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heptachlor, a chlorinated hydrocarbon insecticide, has been considered an environmental contaminant with potential adverse health effects. Exposure to heptachlor may impair immune functions including the inhibition of leukocyte chemotaxis, which causes defects in host defense mechanisms. This study addresses the effects of heptachlor on the cell cycle progression of human lymphocytes. It has been found that addition of heptachlor to cultured lymphocytic cells prevents the cells from progression into the S phase of the cell cycle, with a concomitant accumulation of cells in G1 phase. An accompanying decrease (deactivation) in cyclin-dependent kinase cdk2 and dephosphorylation (activation) of cdc2 was observed. The altered cell cycle progression may trigger the cell's apoptotic potential, as indicated by the reduced amount of Bcl-2 synthesized inside heptachlor-treated cells. The interference of cell cycle progression by heptachlor was also seen with chlordane and toxaphene, two other chlorinated hydrocarbon insecticides.
Authors:
L F Chuang; S E Rought; R Y Chuang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  13     ISSN:  0258-851X     ISO Abbreviation:  In Vivo     Publication Date:    1999 Nov-Dec
Date Detail:
Created Date:  2000-06-21     Completed Date:  2000-06-21     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  455-61     Citation Subset:  IM    
Affiliation:
Department of Medical Pharmacology and Toxicology, School of Medicine, University of California, Davis 95616, USA. rychuang@ucdavis.edu
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / immunology
Blotting, Western
CDC2 Protein Kinase / analysis,  biosynthesis*
CDC2-CDC28 Kinases*
Cell Line
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinases / analysis,  biosynthesis*
Flow Cytometry
G1 Phase / drug effects
Heptachlor / pharmacology*
Humans
Insecticides / pharmacology*
Lymphocytes / cytology,  drug effects*,  enzymology*
Protein-Serine-Threonine Kinases / analysis,  biosynthesis*
S Phase / drug effects
Signal Transduction / drug effects
Toxaphene / pharmacology
Grant Support
ID/Acronym/Agency:
DA 05901/DA/NIDA NIH HHS; DA 10433/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Insecticides; 76-44-8/Heptachlor; 8001-35-2/Toxaphene; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/CDK2 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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