Document Detail

Differential regulation of lymphoproliferative responses to Trypanosoma cruzi antigen in patients with the cardiac or indeterminate form of Chagas disease.
MedLine Citation:
PMID:  15093563     Owner:  NLM     Status:  MEDLINE    
In the search to identify differences in the immunological response between patients with the indeterminate or cardiac form of Chagas disease, trypomastigote-specific peripheral blood mononuclear cell (PBMC) proliferative responses were studied. Suppression of lymphoproliferation occurred in both groups of patients, being more intense in those with the cardiac form. By adding to the cultures neutralizing mAbs anti-IFN-gamma, anti-IL-4, anti-IL-13, or anti-IL-10, indomethacin to block prostaglandin synthesis, NMMA as inhibitor of nitric oxide (NO) synthesis, or glutathione-peroxidase as H(2)O(2) scavenger, it was found that indomethacin augmented lymphoproliferation in both groups of patients. However, anti-IL-10 treatment increased proliferation only in PBMC cultures from patients with the cardiac form, indicating that in these patients, IL-10 was suppressing the immune response. These patients also had higher IL-10 levels in unstimulated cultures and higher PGE(2) levels in stimulated cultures. The results evidence that IL-10 regulates parasite-specific T cell responses in patients with the cardiac form, whereas regulation by prostaglandins (PG) occurs in patients with either cardiac or indeterminate form of the disease.
Sílvia de Barros-Mazon; Maria E Guariento; Cleide Aparecida da Silva; Robert L Coffman; Ises A Abrahamsohn
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical immunology (Orlando, Fla.)     Volume:  111     ISSN:  1521-6616     ISO Abbreviation:  Clin. Immunol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-19     Completed Date:  2004-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883537     Medline TA:  Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  137-45     Citation Subset:  IM    
Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, 13083-970, SP, Brazil.
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MeSH Terms
Antigens, Protozoan / immunology*
Chagas Disease / immunology*
Cytokines / immunology
Dinoprostone / immunology
Interleukin-10 / immunology
Leukocytes, Mononuclear / immunology*,  parasitology
Lymphocyte Activation / immunology
Middle Aged
Trypanosoma cruzi / immunology*
Reg. No./Substance:
0/Antigens, Protozoan; 0/Cytokines; 130068-27-8/Interleukin-10; 363-24-6/Dinoprostone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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