| Differential regulation of the interferon induced gene ISG12A by serum from healthy and preeclamptic pregnancies. | |
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MedLine Citation:
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PMID: 18329281 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endothelial-cell dysfunction is central in the preeclamptic pathogenesis. Several components present in the blood of the preeclamptic mother are capable of mediating this dysfunction. We analyzed the regulation of the ISG12A gene by serum from the third trimester, to elucidate the role of type 1 interferon ISGs late in both healthy and preeclamptic pregnancies. The ISG12A transcription was up-regulated by serum from healthy pregnant women, but not by preeclamptic serum in HeLa and human umbilical vein endothelial cells (HUVEC). However, the ISG12A up-regulation by healthy pregnancy serum was not due to a general type 1 interferon response, since 6-16 and OAS1 were not up-regulated similarly. Also, the up-regulation of ISG12A was independent of the interferon-alpha receptor 2, but dependent on STAT1. Stimulation with folic acid alone or in combination with preeclamptic serum up-regulated ISG12A and 6-16. We conclude that type 1 interferon is not increased in third trimester serum, neither from healthy nor preeclamptic pregnancies. However, since ISG12A mRNA is up-regulated in healthy pregnancies, the ISG12A protein might take part in maintaining endothelial stability, as this function is lacking in preeclamptic pregnancies. Folic acid may ameliorate endothelial cell stability in preeclampsia by up-regulating ISG12A. |
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Authors:
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Camilla S Kronborg; Ulla B Knudsen; Pia M Martensen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-07 |
Journal Detail:
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Title: Cytokine Volume: 42 ISSN: 1096-0023 ISO Abbreviation: Cytokine Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-04-02 Completed Date: 2008-09-04 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9005353 Medline TA: Cytokine Country: United States |
Other Details:
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Languages: eng Pagination: 105-12 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, University of Aarhus, C.F. Mollers Allé, blgd. 1130, 8000 Aarhus C, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Endothelial Cells / cytology Female Gene Expression Regulation* Hela Cells Humans Interferon Type I / metabolism* Membrane Proteins / blood*, genetics Pre-Eclampsia* / blood, genetics Pregnancy Promoter Regions, Genetic Signal Transduction / physiology |
| Chemical | |
Reg. No./Substance:
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0/IFI27 protein, human; 0/Interferon Type I; 0/Membrane Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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