Document Detail

Differential regulation of the interferon induced gene ISG12A by serum from healthy and preeclamptic pregnancies.
MedLine Citation:
PMID:  18329281     Owner:  NLM     Status:  MEDLINE    
Endothelial-cell dysfunction is central in the preeclamptic pathogenesis. Several components present in the blood of the preeclamptic mother are capable of mediating this dysfunction. We analyzed the regulation of the ISG12A gene by serum from the third trimester, to elucidate the role of type 1 interferon ISGs late in both healthy and preeclamptic pregnancies. The ISG12A transcription was up-regulated by serum from healthy pregnant women, but not by preeclamptic serum in HeLa and human umbilical vein endothelial cells (HUVEC). However, the ISG12A up-regulation by healthy pregnancy serum was not due to a general type 1 interferon response, since 6-16 and OAS1 were not up-regulated similarly. Also, the up-regulation of ISG12A was independent of the interferon-alpha receptor 2, but dependent on STAT1. Stimulation with folic acid alone or in combination with preeclamptic serum up-regulated ISG12A and 6-16. We conclude that type 1 interferon is not increased in third trimester serum, neither from healthy nor preeclamptic pregnancies. However, since ISG12A mRNA is up-regulated in healthy pregnancies, the ISG12A protein might take part in maintaining endothelial stability, as this function is lacking in preeclamptic pregnancies. Folic acid may ameliorate endothelial cell stability in preeclampsia by up-regulating ISG12A.
Camilla S Kronborg; Ulla B Knudsen; Pia M Martensen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-07
Journal Detail:
Title:  Cytokine     Volume:  42     ISSN:  1096-0023     ISO Abbreviation:  Cytokine     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-02     Completed Date:  2008-09-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  105-12     Citation Subset:  IM    
Department of Molecular Biology, University of Aarhus, C.F. Mollers Allé, blgd. 1130, 8000 Aarhus C, Denmark.
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MeSH Terms
Cells, Cultured
Endothelial Cells / cytology
Gene Expression Regulation*
Hela Cells
Interferon Type I / metabolism*
Membrane Proteins / blood*,  genetics
Pre-Eclampsia* / blood,  genetics
Promoter Regions, Genetic
Signal Transduction / physiology
Reg. No./Substance:
0/IFI27 protein, human; 0/Interferon Type I; 0/Membrane Proteins

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