Document Detail

Differential regulation of inhibin subunits by germ cells in human testes.
MedLine Citation:
PMID:  15551748     Owner:  NLM     Status:  MEDLINE    
Inhibin B is comprised of two dissimilar disulfide-linked subunits, termed alpha and betaB, and is physiologically more important than inhibin A in the male. The aim of this study was to investigate testicular expression of inhibin subtypes in infertile men to uncover any interaction between Sertoli cells and germ cells. Ten azoospermic patients with Sertoli cell only syndrome (SCO) and 39 oligozoospermic men were included in this study. Follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone concentrations were determined by chemiluminescence assays. The serum concentrations of inhibin B were measured by enzyme-linked immunosorbent assay. Immunohistochemical staining for the alpha-subunit, betaA-subunit, and betaB-subunit of inhibin were performed on testicular biopsy specimens. The results were that serum inhibin B was undetectable in azoospermic men with SCO, while it was 133.8 +/- 82.0 pg/ml in oligozoospermic men. There was little expression of betaA in the testes of any patient. Expression of inhibin alpha and betaB was observed in Sertoli cells. The percentage of Sertoli cells expressing inhibin alpha was similar in azoospermic patients with SCO (55.3% +/- 20.6%) and in oligozoospermic patients (42.8% +/- 30.4%). In contrast, expression of betaB in Sertoli cells of azoospermic patients (24.9% +/- 16.8%) was lower than in oligozoospermic men (43.4% +/- 25.5%: P = 0.0308). There are no significant correlations between testicular expression of inhibin betaB and the serum inhibin B concentrations. The expression of inhibin betaB by Sertoli cells is dependent on the coexistence of spermatogenic activity within these seminiferous tubules, explaining why the level of inhibin B is low in patients with SCO.
M Fujisawa; T Yamazaki; H Fujioka; A Takenaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of andrology     Volume:  50     ISSN:  0148-5016     ISO Abbreviation:  Arch. Androl.     Publication Date:    2004 Sep-Oct
Date Detail:
Created Date:  2004-11-19     Completed Date:  2005-02-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806755     Medline TA:  Arch Androl     Country:  England    
Other Details:
Languages:  eng     Pagination:  339-45     Citation Subset:  IM    
Department of Urology, Kawasaki Medical School, National Kobe Hospital, Kurashiki, Japan.
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MeSH Terms
Follicle Stimulating Hormone / blood
Hormones / blood
Inhibin-beta Subunits / analysis,  metabolism*
Inhibins / analysis,  metabolism*
Luteinizing Hormone / blood
Oligospermia / blood,  pathology*
Sertoli Cells / pathology*
Spermatozoa / pathology*
Testis / pathology*
Reg. No./Substance:
0/Hormones; 0/INHBB protein, human; 0/inhibin-alpha subunit; 57285-09-3/Inhibins; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone; 93443-12-0/Inhibin-beta Subunits

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