| Differential regulation of monocyte/macrophage cytokine production by pressure. | |
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MedLine Citation:
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PMID: 16226954 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Cytokine production by macrophages is essential for the inflammatory response. Normal human interstitial tissue pressure is 20 to 30 mm Hg, but generally decreases in acute inflammation. METHODS: We compared the effect of 20 mm Hg increased pressure (approximating normal interstitial tissue pressure) with that of ambient pressure (resembling pressure in inflamed tissues) on tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta production by undifferentiated (monocytic) and PMA (phorbol 12-, myristate 13-acetate)-differentiated (macrophage-like) THP-1 cells with or without lipopolysaccharide (LPS) (10 ng/mL). RESULTS: Pressure stimulated spontaneous macrophage TNF-alpha secretion (30.5 +/- 6.3 vs. 49.1 +/- 2.8 pg/mL, P <.02), but not monocyte TNF-alpha secretion. Pressure did not stimulate IL-1beta release. As expected, LPS increased basal cytokine release. After LPS stimulation, pressure still tended to stimulate macrophage TNF-alpha, but inhibited monocyte TNF-alpha secretion (P <.05). In contrast, pressure inhibited IL-1beta release by both LPS-treated monocytes (986 +/- 134 vs. 595 +/- 226 pg/mL, P <.02) and macrophages (3,112 +/- 229 vs. 979 +/- 61 pg/mL, P <.01). CONCLUSIONS: Extracellular pressure may regulate TNF-alpha and IL-1beta secretion differentially by monocytes and macrophages. |
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Authors:
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Hiroe Shiratsuch; Marc D Basson |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of surgery Volume: 190 ISSN: 0002-9610 ISO Abbreviation: Am. J. Surg. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-10-17 Completed Date: 2005-11-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370473 Medline TA: Am J Surg Country: United States |
Other Details:
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Languages: eng Pagination: 757-62 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, Wayne State University, Detroit, MI, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Enzyme-Linked Immunosorbent Assay Extracellular Space / physiology* Humans Interleukin-1 / secretion* Macrophages / metabolism* Monocytes / metabolism* Pressure Tumor Necrosis Factor-alpha / secretion* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK60771/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1; 0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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