| Differential regulation of collagen degradation by rabbit keratocytes and polymorphonuclear leukocytes. | |
| | |
MedLine Citation:
|
PMID: 12221535 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: Both activated keratocytes and infiltrated polymorphonuclear leukocytes (PMNs) contribute to corneal ulceration by degrading stromal collagen. The regulation of such collagen degradation by inflammatory cytokines was investigated with rabbit keratocytes and PMNs cultured in three-dimensional collagen gels. METHODS: Rabbit keratocytes or PMNs were cultured for 24 h in three-dimensional gels of type I collagen in the presence of plasminogen and various concentrations of either interleukin (IL)-1alpha, IL-6, IL-8, or tumor necrosis factor-alpha (TNF-alpha). Degradation of collagen during culture was assessed by measurement of released hydroxyproline. RESULTS: IL-1alpha increased the amount of collagen degraded by keratocytes or PMNs in a dose-dependent manner, whereas IL-6 had no effect on collagen degradation by either cell type. IL-8 increased the extent of collagen degradation by PMNs but not that by keratocytes, and TNF-alpha promoted collagen degradation by keratocytes but not that by PMNs. CONCLUSION: Inflammatory cytokines regulate collagen degradation by rabbit keratocytes and PMNs in culture in a differential manner, and therefore may contribute to the roles of these cells in corneal ulceration. |
| | |
Authors:
|
Takashi Nagano; Masatsugu Nakamura; Teruo Nishida |
Related Documents
:
|
15661925 - A chemotactic peptide from laminin alpha 5 functions as a regulator of inflammatory imm... 16157315 - The role of rac1 and rac2 in bacterial killing. 12237255 - Pharmacological analysis for mechanisms of gpi-80 release from tumour necrosis factor-a... 10102555 - Polyamines found in the inflamed periodontium inhibit priming and apoptosis in human po... 16351945 - Formation of the corneous layer in the epidermis of the tuatara (sphenodon punctatus, s... 15033785 - Effects of membrane attack complex of complement on apoptosis in experimental autoimmun... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Current eye research Volume: 24 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 2002 Mar |
Date Detail:
|
Created Date: 2002-09-10 Completed Date: 2002-12-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
|
Languages: eng Pagination: 240-3 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology, Yamaguchi University School of Medicine, Ube, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Count Cells, Cultured Collagen / metabolism* Cornea / cytology, drug effects, metabolism* Dose-Response Relationship, Drug Interleukin-1 / pharmacology Interleukin-6 / pharmacology Interleukin-8 / pharmacology Male Neutrophils / cytology, drug effects, metabolism* Plasminogen / pharmacology Rabbits Tumor Necrosis Factor-alpha / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Interleukin-1; 0/Interleukin-6; 0/Interleukin-8; 0/Tumor Necrosis Factor-alpha; 9001-91-6/Plasminogen; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A histological analysis of the protective effect of ischemic preconditioning in the rat retina.
Next Document: Genetic and molecular characterization of 148 patients with autosomal dominant retinitis pigmentosa ...