| Differential regulation by fucoidan of IFN-γ-induced NO production in glial cells and macrophages. | |
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MedLine Citation:
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PMID: 20830678 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Fucoidan has shown numerous biological actions; however, the molecular bases of these actions have being issued. We examined the effect of fucoidan on NO production induced by IFN-γ and the molecular mechanisms underlying these effects in two types of cells including glia (C6, BV-2) and macrophages (RAW264.7, peritoneal primary cells). Fucoidan affected IFN-γ-induced NO and/or iNOS expression both in macrophages and glial cells but in a contrast way. Our data showed that in C6 glioma cells both JAK/STAT and p38 signaling positively regulated IFN-γ-induced iNOS, which were inhibited by fucoidan. In contrast, in RAW264.7 cells JAK/STAT is a positive regulator whereas p38 is a negative regulator of NO/iNOS production. In RAW264.7 cells, fucoidan enhanced p38 activation and induced TNF-α production. We also confirmed the dual regulation of p38 in BV-2 microglia and primary peritoneal macrophages. From these results, we suggest that fucoidan affects not only IFN-γ-induced NO/iNOS production differently in brain and peritoneal macrophages due to the different roles of p38 but the effects on TNF-α production in the two cell types. These novel observations including selective and cell-type specific effects of fucoidan on IFN-γ-mediated signaling and iNOS expression raise the possibility that it alters the sensitivity of cells to the p38 activation. |
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Authors:
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Hang Do; Nam-Sung Kang; Suhkneung Pyo; Timothy R Billiar; Eun-Hwa Sohn |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 111 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1337-45 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Wiley-Liss, Inc. |
Affiliation:
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Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University, Kyunggi-do 440-746, Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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