| Differential regulation of Toll-like receptor signalling in spleen and Peyer's patch dendritic cells. | |
| | |
MedLine Citation:
|
PMID: 20545785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Toll-like receptor (TLR) signalling shapes dendritic cell (DC) responses by inducing co-stimulatory molecule up-regulation and cytokine secretion while TLR regulatory proteins inhibit this process. We aimed to determine if gene expression of TLRs and TLR regulatory proteins underpins the functionally different lipopolysaccharide (LPS) responses of DCs from murine Peyer's patches (PP) and spleen and of murine bacteria-conditioned bone-marrow-derived cells. Isolated spleen and PP DCs were analysed for basal expression of TLRs by flow cytometry and real time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The DCs were stimulated with LPS to determine cytokine secretion by enzyme-linked immunosorbent assay and expression of TLR regulatory proteins by qRT-PCR. In vitro results were confirmed following in vivo intraperitoneal LPS injection. In addition, changes in gene expression of TLR regulatory proteins were assessed in bacteria-conditioned bone-marrow-derived cells. Results indicated that surface expression of TLR2 and TLR4 on PP DCs was decreased compared with spleen DCs. The PP DCs secreted a limited profile of cytokines compared with spleen DCs following LPS stimulation. In vivo LPS exposure up-regulated sigirr, tollip and tmed1 messenger RNA in PP DCs, but not spleen DCs. Similar gene expression changes were observed in bacteria-conditioned bone-marrow-derived cells. Therefore, functionally different LPS responses in PP and spleen DCs reflect their characteristic expression of TLRs and TLR regulatory proteins. Differential regulation of TLR signalling was also evident in bacteria-conditioned bone-marrow-derived cells indicating that bacterial signalling may be a mechanism for inducing altered gene regulation in PP DCs. |
| | |
Authors:
|
Julie M Davies; John MacSharry; Fergus Shanahan |
Related Documents
:
|
16583355 - Dc-sign association with the th2 environment of lepromatous lesions: cause or effect? 18023445 - Tick saliva inhibits the chemotactic function of mip-1alpha and selectively impairs che... 20607865 - Effects of axon degeneration on oligodendrocyte lineage cells: dorsal rhizotomy evokes ... 15762875 - Regulation of dendritic cell interleukin-12 secretion by tumour cell necrosis. 21756955 - Hiv-1 gp120 induces antioxidant response element-mediated expression in primary astrocy... 16757035 - Down-regulation of t cell responses to achr and reversal of eamg manifestations in mice... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Immunology Volume: 131 ISSN: 1365-2567 ISO Abbreviation: Immunology Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-11-02 Completed Date: 2011-02-02 Revised Date: 2011-11-01 |
Medline Journal Info:
|
Nlm Unique ID: 0374672 Medline TA: Immunology Country: England |
Other Details:
|
Languages: eng Pagination: 438-48 Citation Subset: IM |
Copyright Information:
|
© 2010 The Authors. Immunology © 2010 Blackwell Publishing Ltd. |
Affiliation:
|
Alimentary Pharmabiotic Centre, National University of Ireland, University College Cork, Cork, Ireland. juliemariedavies@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bifidobacteriales Infections / immunology* Bifidobacterium / immunology*, pathogenicity Bone Marrow Cells / immunology, metabolism*, microbiology, pathology Cells, Cultured Cytokines / secretion Dendritic Cells / immunology, metabolism*, pathology Gene Expression Regulation Intracellular Signaling Peptides and Proteins / genetics, metabolism Lipopolysaccharides / immunology Mice Mice, Inbred BALB C Peyer's Patches / pathology* Receptors, Interleukin-1 / genetics, metabolism Signal Transduction Spleen / pathology* Toll-Like Receptor 2 / immunology, metabolism* Toll-Like Receptor 4 / immunology, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Intracellular Signaling Peptides and Proteins; 0/Lipopolysaccharides; 0/Receptors, Interleukin-1; 0/Tir8 protein, mouse; 0/Tlr2 protein, mouse; 0/Tlr4 protein, mouse; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 0/Tollip protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Natural killer T cells constitutively expressing the interleukin-2 receptor ? chain early in life ar...
Next Document: Unsweetened ice popsicles impart a positive feeling and reduce self-mutilation after paediatric dent...