|Differential regulation of HIF-mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts.|
|Jump to Full Text|
|PMID: 23303559 Owner: NLM Status: MEDLINE|
|Inappropriate osteoclast activity instigates pathological bone loss in rheumatoid arthritis. We have investigated how osteoclasts generate sufficient ATP for the energy-intensive process of bone resorption in the hypoxic microenvironment associated with this rheumatic condition. We show that in human osteoclasts differentiated from CD14(+) monocytes, hypoxia (24 h, 2% O2 ): (a) increases ATP production and mitochondrial electron transport chain activity (Alamar blue, O2 consumption); (b) increases glycolytic flux (glucose consumption, lactate production); and (c) increases glutamine consumption. We demonstrate that glucose, rather than glutamine, is necessary for the hypoxic increase in ATP production and also for cell survival in hypoxia. Using siRNA targeting specific isoforms of the hypoxia-inducible transcription factor HIF (HIF-1α, HIF-2α), we show that employment of selected components of the HIF-1α-mediated metabolic switch to anaerobic respiration enables osteoclasts to rapidly increase ATP production in hypoxia, while at the same time compromising long-term survival. We propose this atypical HIF-driven metabolic pathway to be an adaptive mechanism to permit rapid bone resorption in the short term while ensuring curtailment of the process in the absence of re-oxygenation.|
|Karl J Morten; Luned Badder; Helen J Knowles|
Related Documents :
|24105869 - Atp-binding cassette transporters in liver.
25378199 - Variable electrobehavioral patterns during focal nonconvulsive status epilepticus induc...
24561259 - Are long chain acyl coas responsible for suppression of mitochondrial metabolism in hib...
25413449 - Glucose metabolism following human traumatic brain injury: methods of assessment and pa...
19421159 - Metabolism control by the circadian clock and vice versa.
24830319 - Effects of thienorphine on contraction of the guinea pig sphincter of oddi, choledochus...
7965739 - A functional beta-2 adrenoceptor-mediated chronotropic response in isolated guinea pig ...
2899009 - Enzymatic histamine catabolism in vertebrate ontogenesis. a comparative study.
8265879 - Ultrastructural study of rabbit lung tissue subsequent to capsaicin treatment over seve...
|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: The Journal of pathology Volume: 229 ISSN: 1096-9896 ISO Abbreviation: J. Pathol. Publication Date: 2013 Apr|
|Created Date: 2013-03-15 Completed Date: 2013-05-02 Revised Date: 2014-02-20|
Medline Journal Info:
|Nlm Unique ID: 0204634 Medline TA: J Pathol Country: England|
|Languages: eng Pagination: 755-64 Citation Subset: IM|
|Copyright © 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.|
|APA/MLA Format Download EndNote Download BibTex|
Adenosine Triphosphate / metabolism*
Basic Helix-Loop-Helix Transcription Factors / genetics, metabolism*
Bone Resorption / genetics, metabolism*, pathology, physiopathology
Electron Transport Chain Complex Proteins / metabolism
Glucose / metabolism
Glutamine / metabolism
Hypoxia-Inducible Factor 1, alpha Subunit / genetics, metabolism*
Lactic Acid / metabolism
Mitochondria / metabolism*
Osteoclasts / metabolism*, pathology
Pyruvate Dehydrogenase Complex / metabolism
|19200//Arthritis Research UK; MP/19200//Arthritis Research UK|
|0/Basic Helix-Loop-Helix Transcription Factors; 0/Electron Transport Chain Complex Proteins; 0/HIF1A protein, human; 0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/Pyruvate Dehydrogenase Complex; 0/endothelial PAS domain-containing protein 1; 0RH81L854J/Glutamine; 33X04XA5AT/Lactic Acid; 8L70Q75FXE/Adenosine Triphosphate; IY9XDZ35W2/Glucose|
Journal ID (nlm-ta): J Pathol
Journal ID (iso-abbrev): J. Pathol
Journal ID (publisher-id): path
Publisher: John Wiley & Sons, Ltd, Chichester, UK
Copyright © 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Received Day: 07 Month: 11 Year: 2012
Revision Received Day: 02 Month: 12 Year: 2012
Accepted Day: 21 Month: 12 Year: 2012
Print publication date: Month: 4 Year: 2013
Electronic publication date: Day: 13 Month: 3 Year: 2013
Volume: 229 Issue: 5
First Page: 755 Last Page: 764
PubMed Id: 23303559
|Differential regulation of HIF-mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts|
|Karl J Morten1|
|Helen J Knowles2*|
1Nuffield Department of Obstetrics and Gynaecology, The Womens Centre, John Radcliffe HospitalOxford, UK
2Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Nuffield Orthopaedic CentreOxford, UK
|Correspondence: *Correspondence to: Helen J Knowles, Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Nuffield Orthopaedic Centre, Oxford OX3 7LD, UK. e-mail: firstname.lastname@example.org
Conflicts of interest: LB received research funding from Luxcel Biosciences, which manufactures MitoXpress-Xtra-HS. KJM and HJK state that they have no conflicts of interest.
Osteoclasts are large, non-proliferative, multinucleated cells responsible for the resorptive component of bone remodelling. Osteoclast-mediated osteolysis is a diagnostic feature of rheumatoid arthritis (RA), contributing to progressive disability and predictive of poor prognosis 1. Osteoclasts bind bone via αvβ3 integrin, then form an F-actin-rich seal to isolate a resorptive compartment above the osseous substrate. Resorption is initiated by active transport of protons across the bone-apposing membrane by vacuolar H+ ATPase, a process also supported by Na,K-ATPase, Ca-ATPase and gastric H,K-ATPase 2. This acidifies the resorptive compartment, exposing the organic matrix to proteolytic enzymes also secreted from the osteoclast. Osteoclasts are additionally highly motile and these processes render bone resorption an energy-intensive process with a high demand for ATP 3,4.
Hypoxia is another feature of the rheumatoid joint and also a poor prognostic indicator 5. Cellular adaptation to hypoxia generally entails switching to anaerobic metabolism; reducing ATP production to prevent accumulation of reactive oxygen species (ROS) 6. This survival mechanism is largely mediated by the hypoxia-inducible factor (HIF-1, HIF-2) family of transcription factors. Post-translational stabilization of the α-subunit of HIF-1 and HIF-2 under hypoxia transactivates numerous downstream genes, including those mediating the switch to initiate metabolic adaptation to hypoxia and maintain energy/redox homeostasis 7.
First, HIF triggers a cytochrome c oxidase subunit switch (COX4-1 to COX4-2), which increases the efficiency of complex IV of the mitochondrial electron transport chain (ETC) with respect to the amounts of ATP and ROS produced 8. Once this is insufficient to maintain homeostasis, HIF stimulates glucose transporter and glycolytic enzyme expression to increase glycolytic flux 9. Third, HIF inhibits pyruvate dehydrogenase (PDH), the mitochondrial enzyme that converts pyruvate into acetyl CoA, by increasing expression of PDH kinase (PDK) which phosphorylates and inactivates PDH 10,11. This reduces flux through the mitochondrial tricarboxylic acid (TCA) cycle and ETC and reduces accumulation of ROS. Fourth, HIF induces expression of BCL2/adenovirus E1B 19 kDa interacting protein 3 (BNIP3), which competes with Beclin-1 for binding to Bcl-2, releasing Beclin-1 to stimulate mitochondrial autophagy and also reduce accumulation of ROS 12.
Hypoxia exerts various effects on osteoclasts. It reduces the viability of mature osteoclasts 13,14, increases osteoclast differentiation when combined with periods of re-oxygenation 13–16 and increases bone resorption in a HIF-1α-dependent manner 13–17. Osteoclasts exhibit elevated expression of TCA cycle and oxidative phosphorylation enzymes 18, high rates of oxygen consumption 19 and numerous mitochondria 20, suggesting that high mitochondrial metabolic activity drives ATP production in these cells. The preferred substrate is glucose (via glycolysis) 19,21,22, although fatty acid oxidation has been implicated 23. Given the high energy requirement for resorption, it could be presumed that increased resorption under hypoxia would increase cellular demand for ATP. However, switching to anaerobic glycolysis would not be expected to support this increased demand.
We have investigated the metabolic requirements of hypoxic osteoclasts to determine how they generate the requisite ATP for increased bone resorption. Identification of key components of this pathway might highlight possible therapeutic targets for amelioration of pathological bone resorption conditions.
Tissue culture reagents were from Lonza (Wokingham, UK), except FBS (Invitrogen, Paisley, UK), M-CSF (R&D Systems, Abingdon, UK) and RANKL (PeproTech, London, UK). Compound C was from Merck (Feltham, UK). Unless stated otherwise, other reagents were from Sigma (Poole, UK). This study was approved by the Oxford Clinical Research Ethics Committee (C01.071) and the Oxford Musculoskeletal BioBank.
Cells were cultured in α-MEM (without ribonucleosides/deoxyribonucleosides), 10% FBS, L-glutamine (2 mM), penicillin (50 IU/ml) and streptomycin sulphate (50 µg/ml). Peripheral blood mononuclear cells were isolated from buffy coat using Histopaque. Positively selected CD14+ monocytes (AutoMACS cell separator; Miltenyi Biotec, Bisley, UK) were seeded onto tissue culture plates, glass slides or dentine slices at 106 cells/well of a 24-well plate. Non-adherent cells were removed and cultures supplemented with (a) M-CSF (25 ng/ml) for monocyte culture (≤ 72 h) or (b) M-CSF (25 ng/ml) + RANKL (50 ng/ml) every 3–4 days for osteoclast culture, with experiments performed on days 13–17. Primary human osteoblasts were obtained by outgrowth from cancellous bone chips removed during surgery. Hypoxic exposure (2% O2, 5% CO2, balance N2) was performed in a MiniGalaxy incubator (RS Biotech, Irvine, UK). Vitronectin receptor (VNR) was detected using a CD51/61 monoclonal antibody (AbD Serotec, Oxford, UK). Multinucleated cells containing ≥ 3 nuclei were considered osteoclasts. Resorption pits were stained (0.5% toluidine blue), photographed and quantified using ImageJ.
Intracellular ATP was assayed using CellTiter Glo (Promega, Southampton, UK). Mitochondrial dehydrogenase activity within the ETC was assessed using Alamar blue (AbD Serotec) and normalized to cell number using crystal violet, which stains nuclei independently of cellular metabolic status. Oxygen consumption was assessed using MitoXpress-Xtra-HS (Luxcel Biosciences, Cork, Ireland), a porphyrin-based phosphorescent oxygen-sensitive probe. 16 h prior to assay, osteoclasts were transferred into fresh culture medium or medium lacking glucose but supplemented with 1 mM pyruvate. Probe (10 µl) was added and the cells equilibrated at 20% or 2% O2. The assay was read using a FLUOstar Omega plate reader with ACU (BMG Labtech, Aylesbury, UK), held at 20% or 2% O2, the maximal rate of oxygen consumption being proportional to the change in probe fluorescence during the linear phase of the assay 24 (see Supplementary material, additional methodology for O2 consumption assay). To assess relative mitochondrial number, nonyl-acridine orange was applied to cells in 5% FBS/PBS (50 nM, 30 min, 37°C), then visualized by fluorescence microscopy. Staining intensity was quantified in ImageJ and normalized to number of nuclei and osteoclast area.
RNA was extracted in TRI reagent, DNase-treated and reverse-transcribed (SuperScript VILO cDNA Synthesis Kit, Invitrogen). Real-time PCR was performed with Express SYBR GreenER qPCR Supermix Universal (Invitrogen) and QuantiTect primers (Qiagen, Crawley, UK). Expression was normalized to ACTB.
Cells were homogenized in lysis buffer [6.2 M urea, 10% glycerol, 5 mM dithiothreitol (DTT), 1% sodium dodecyl sulphate (SDS), protease inhibitors] or phospho-lysis buffer [1 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 1 mM Na3VO4, 1 mM NaF in PBS]. Primary antibodies were against SOD2 (ab13533), porin (clone 31HL; Abcam, Cambridge, UK), BNIP3 (clone Ana40), COX IV isoform 1, COX IV isoform 2 (Novus Biologicals, Cambridge, UK), HIF-1α (clone 54; BD Biosciences), AMPKα (23A3), phospho-AMPKα (Thr172, 40H9; Cell Signalling Technology, Danvers, MA, USA) and β-tubulin (TUB2.1).
Glucose and glutamine were measured using the Glucose (GO) Assay Kit and Glutamine/Glutamate Determination Kit. Lactate was assayed in heat-inactivated medium by the increase in absorbance (340 nm) as NAD+ was converted to NADH in the presence of 0.32 M glycine, 0.32 M hydrazine, 9.6 mM NAD+ and 3 U/ml lactate dehydrogenase. The results were normalized to osteoclast number. Intracellular neutral lipid was detected in formalin-fixed cells washed with 60% isopropanol, air-dried, incubated in Oil Red O (70 mM) and washed and photographed in distilled water.
The PDH Enzyme Activity Microplate Assay (Abcam) was used to assess the ability of immunocaptured PDH to convert pyruvate to acetyl-CoA by following the reduction of NAD+ to NADH 17.
Mature osteoclasts were transfected (RNAiMAX, Invitrogen) with 50 nM siRNA targeting HIF-1α, HIF-2α or an HIF-1α scrambled control. Duplexes were removed after 16 h and osteoclasts incubated for a further 8 h prior to hypoxic stimulation, achieving 75 ± 4% (HIF-1α) and 49 ± 6% (HIF-2α) protein inhibition, as described 17.
Osteoclasts were transfected with PGK HRE–firefly luciferase (a gift from Professor AL Harris, Oxford, UK) and pHRG–TK Renilla luciferase plasmids (Promega), using Lipofectamine 2000 (Invitrogen). 16 h post-transfection, the cells were exposed to experimental conditions. Luminesence was assayed using the Dual-Luciferase Reporter Assay System (Promega), with firefly luciferase normalized to the Renilla transfection control.
Results are expressed as mean ± standard deviation (SD) of at least three independent experiments. Statistical analysis comprised one-way analysis of variance (ANOVA) using Bonferroni's multiple comparison as a post hoc test (except for experiments with only two conditions, for which a t-test was applied), with results considered significant at p < 0.05.
To investigate whether hypoxic osteoclasts produce additional energy for bone resorption, we measured intracellular ATP under normoxia and hypoxia (24 h, 2% O2). When cultured on plastic, primary monocytes and osteoblasts, which share the osteoclast bone micro-environment, showed reduced intracellular ATP in line with published reports 6, whereas hypoxic osteoclasts increased intracellular ATP by 56% (Figure 1A). When cultured instead on dentine, a substrate on which osteoclast resorption mechanisms are active, the hypoxic increase in intracellular ATP was not evident, suggesting that this ATP is utilized for bone resorption (Figure 1A).
We next assessed mitochondrial metabolic flux, assaying ETC activity using Alamar Blue 25. Hypoxic osteoclasts rapidly increased ETC activity (125%, 4 h), reaching 169% at 24 h compared with decreased ETC activity in monocytes and osteoblasts (Figure 1B). Unaltered mitochondrial porin expression (Figure 1C) and nonyl-acridine orange staining (which binds mitochondrial cardiolipin; data not shown), suggested this was not due to increased mitochondrial mass. O2 consumption remained considerable under hypoxia; indeed, ETC inhibition with rotenone had a greater effect under hypoxia (74% reduction) than in normoxic conditions (44% reduction; Figure 1D). In both environments O2 consumption via the ETC. remained close to maximal, as compared with cells cultured in supplementary pyruvate (Figure 1D). However, as probe sensitivity to changes in O2 concentration is greater in the low O2 range, we were unable to compare O2 consumption rates at 20% and 2% O2 directly.
HIF-1α siRNA reduced the hypoxic increase in ETC activity by 25% (Figure 2A), suggesting it to be partially HIF-1α-mediated. We therefore considered mitochondrial components of the HIF-mediated metabolic switch. COX subunit switching was evident, with increased COX4-2 (mRNA, protein) and reduced COX4-1 protein due to induction of the mitochondrial protease LONP1 (Figure 2B, C). However, despite hypoxic induction of BNIP3 mRNA, BNIP3 protein was unchanged (Figure 2B, C). This implies no stimulation of mitophagy, despite reduced expression of mitochondrial mRNAs, ATP synthase F0 subunit a/8 (ATP6/8) and cytochrome c oxidase subunit 3 (CO3) (Figure 2B).
Glycolysis is the major pathway driving osteoclast metabolism. Hypoxia increased glucose transporter (Glut-1, SLC2A1) and glycolytic enzyme mRNA expression (Figure 3A). Hypoxic transactivation of the PGK-1 hypoxia response element was HIF-1α-dependent, while HIF-2α exerted a small inhibitory effect (Figure 3B). Hypoxia increased glucose uptake by osteoclasts (and primary monocytes and osteoblasts) in a HIF-1α-dependent manner (Figure 3C, D). Hypoxia increased osteoclast lactate secretion, although the ratio of glucose consumption to lactate production was unaltered (Figure 3C), indicative of increased flux through the glycolytic pathway but not of a switch to anaerobic glycolysis.
The PDH complex converts pyruvate to acetyl CoA and is permissive for continued mitochondrial metabolic flux. Phosphorylation by the HIF-1α target gene PDK1 inhibits PDH activity. Despite hypoxic induction of PDK1 mRNA (two-fold; p < 0.05), no consistent effect of hypoxia was observed on PDK1 protein expression in osteoclasts (data not shown). Hypoxia did not alter PDH activity (Figure 4A) and neither did isoform-specific HIF siRNA affect PDH activity in either condition (data not shown).
We therefore considered whether another regulator of cellular energy status, AMP-activated protein kinase (AMPK), might regulate PDH activity in osteoclasts. AMPK is activated by phosphorylation of Thr172 under metabolically stressful conditions, such as hypoxia 26. However, AMPK phosphorylation was almost ablated in hypoxic osteoclasts (Figure 4B). Chemical inhibition of AMPK with compound C similarly did not affect PDH activity (Figure 4C, D). However, chemical activation of AMPK with metformin inhibited PDH, suggesting that osteoclast AMPK could inhibit PDH activity under hypoxia if it were activated.
We next considered other substrates of mitochondrial metabolism that might contribute to hypoxic ATP generation. Fatty acid oxidation has been proposed to drive ATP production in osteoclasts 23. However, hypoxic osteoclasts accumulated intracellular neutral lipid (Figure 5A), suggesting that hypoxia inhibits mitochondrial import and/or utilization of fatty acids 27,28. Hypoxia did not affect lipid accumulation in monocytes or osteoblasts.
Glutamine, a substrate for glutaminolysis, enters the mitochondrial TCA cycle at α-ketoglutarate. Hypoxic osteoclasts increased glutamine uptake 4.1-fold, whereas osteoblasts and monocytes inhibited and maintained uptake respectively (Figure 5B). Hypoxic induction of glutamate uptake was HIF-2α-dependent (Figure 5C). However, glutamine withdrawal had no effect on either ATP production or osteoclast survival, whereas removal of glucose dramatically inhibited both, especially in hypoxia (Figure 5D).
In contrast to other cells, osteoclasts are sensitive to even moderate hypoxia. 2% O2 reduced osteoclast numbers to 83% and 65% of normoxic levels at 24 and 72 h respectively, whereas primary monocytes and osteoblasts continued to proliferate (Figure 6A). We previously hypothesized the extent of osteoclast-mediated bone resorption under hypoxia to be a balance between osteoclast activation and osteoclast apoptosis 14. Sensitivity to hypoxia-induced cell death might be a consequence of maintaining high rates of oxidative phosphorylation in a hypoxic environment, evidenced by increased expression of superoxide dismutase 2 (SOD2) (Figure 6B), a marker of mitochondrial ROS formation. HIF-1α siRNA completely rescued osteoclasts from cell death induced by chronic (48 h) hypoxic exposure (Figure 6C), whereas modulation of AMPK activity had no effect (Figure 6D).
We have demonstrated that hypoxic osteoclasts increase flux through both the glycolytic pathway and the mitochondrial ETC, driven by employment of selected components of the HIF-1α-mediated switch to anaerobic glycolysis. This enables osteoclasts to generate sufficient ATP to support enhanced bone resorption.
Glucose regulates osteoclast activity at normal physiological concentrations 21. Increased glucose rapidly elevates the intracellular ATP:ADP ratio 22, while longer exposure activates vacuolar H+-ATPase (V-ATPase) A-subunit transcription to stimulate resorption 29. This subunit directly interacts with the glycolytic enzyme phosphofructokinase-1 (PFK-1) 30. Interaction between aldolase and the V-ATPase E-subunit also occurs in osteoclasts 31. Both interactions are deemed mechanisms to micro-compartmentalize glycolytic ATP generation at the required intracellular location, directly linking glycolysis and osteoclast activation. Increased glycolysis by actively resorbing, hypoxic osteoclasts may also occur in vivo. Positron emission tomography (PET) with 2-(fluorine-18)fluoro-2-deoxy-D-glucose (18FDG) can distinguish benign primary bone tumours containing many osteoclasts from those where osteoclasts are sparse 32, additionally correlating with markers of hypoxia 33. Agents which inhibit glucose uptake/glycolysis inhibit bone resorption in animal models of disease 34,35 and can induce clinical remission in RA 36, suggesting targeted inhibition of glycolysis as a therapeutic anti-resorptive option. However, other cells within the hypoxic rheumatoid joint exhibit increased glycolytic capacity and the inhibitory activity of these agents does not solely target glycolysis, necessitating further investigation of the mechanism(s) behind such results.
Glutamine consumption has not previously been described in osteoclasts. Hypoxia increased glutamine uptake, as in SK–N–SH neuroblastoma 37 and A549 lung adenocarcinoma cells 38, in a HIF-2α-dependent manner. HIF-2α also drives the switch to hypoxic use of glutamine, rather than glucose, as the substrate for lipid synthesis 38. Glutamine is additionally required for nucleotide and hexosamine biosynthesis. This complements our data that hypoxic osteoclasts require glucose for ATP production and cell survival but are insensitive to glutamine withdrawal, suggesting glucose as the primary substrate for ATP production, with glutamine potentially used for biosynthesis.
Osteoclasts only utilized selected components of the classical HIF-1α-mediated metabolic switch to anaerobic respiration. HIF-1α increased hypoxic glucose uptake and glycolytic flux and the HIF-1α-dependent COX subunit switch was observed. However, PDH was not inhibited and BNIP3 expression did not increase. These latter processes normally reduce hypoxic flux through the mitochondrial TCA cycle and ETC 10,11 and induce mitochondrial autophagy 12 respectively, both of which prevent toxic accumulation of ROS. As a result, mitochondrial ROS accumulates in hypoxic osteoclasts. However, ROS are essential for resorption. Tartrate-resistant acid phosphatase (TRAP) and cathepsin K co-localize in transcytotic vesicles. Digestion of TRAP by cathepsin K activates the ROS-generating activity of TRAP to aid degradation of the products of bone digestion 39. Osteoclast differentiation and resorption are enhanced by exogenous inducers of ROS; including H2O240, homocysteine 41 and hypoxia 42. Indeed, this effect of hypoxia requires mitochondrial ROS and is reversed by the mitochondria-specific antioxidant MitoQ 42.
It is unclear why PDH was not inhibited in hypoxic osteoclasts. Despite HIF-1α induction, PDK1 mRNA was only modestly induced and we observed no consistent effect on PDK1 protein. PDH is also inhibited by hypoxic activation of AMPK and induction of PDK4 43. However, in osteoclasts hypoxia inhibited AMPK phosphorylation and so inactivated AMPK. AMPK is usually activated by reduced intracellular ratios of ATP:ADP or ATP:AMP, although hypoxic activation occurs independently of changes in intracellular energy status 26,44. It may be that enhanced ATP production in hypoxic osteoclasts increases the intracellular ratio of ATP:AMP and overrides hypoxic mechanism(s) of AMPK activation in favour of dephosphorylation and inactivation. This would be necessary for hypoxic resorption to proceed, as AMPK inhibits osteoclast differentiation and activation 45. Hypoxic attenuation of PDH activity is therefore prevented in osteoclasts by blockade of at least two pathways that usually contribute to its inhibition, allowing continued mitochondrial metabolic flux under hypoxia.
HIF-1α did not reduce mitochondrial metabolic flux, but did increase glycolytic flux and maintain the COX subunit switch, supporting our observation that increased hypoxic mitochondrial activity is partially HIF-1α-dependent. HIF-1α siRNA also prevented cell death during prolonged hypoxia. We hypothesize that in hypoxic osteoclasts, functional HIF-1α-dependent pathways initially increase ATP production and bone resorption 14. However, lack of activation of HIF-1α-dependent survival pathways eventually results in cell death. As osteoclasts are anyway short-lived cells, and as resorption cannot be allowed to continue indefinitely, allowing progressive accumulation of ROS in hypoxic osteoclasts may be an adaptive mechanism permitting rapid bone resorption in the short term, while ensuring curtailment of the process in the absence of re-oxygenation.
In summary, we have shown that hypoxia stimulates osteoclasts' consumption of glucose and glutamine and that increased glucose uptake is required for increased ATP production. HIF-1α-dependent stimulation of glucose uptake and glycolysis, in the absence of PDH inhibition by either HIF-1α or AMPK, drives high hypoxic mitochondrial ETC activity in these cells, although this eventually results in cell death. These mechanisms appear to support hypoxic induction of osteoclast resorption in the short term and might provide therapeutic targets for amelioration of the pathological bone resorption associated with diseases such as rheumatoid arthritis.
This study was funded by Arthritis Research UK (Grant No. MP/19200). HJK also acknowledges support from the Oxford NIHR BRU. LB is supported by a student stipend from Luxcel Biosciences. We thank Dr James Hynes and Dr Conn Carey from Luxcel Biosciences for technical support and Professor AL Harris (Oxford, UK) for the PGK HRE–firefly luciferase reporter construct.
HJK conceived the study and drafted the manuscript; HJK and KJM designed the study and revised manuscript content; and HJK and LB performed experiments and collected data. All authors were involved in data analysis, data interpretation and approving the final version of the manuscript.
|1.||Gough AK,Lilley J,Eyre S,et al. Generalised bone loss in patients with early rheumatoid arthritisLancetYear: 1994344:23277912297|
|2.||Francis MJ,Lees RL,Trujillo E,et al. ATPase pumps in osteoclasts and osteoblastsInt J Biochem Cell BiolYear: 200234:45947611906818|
|3.||Vaananen HK,Karhukorpi EK,Sundquist K,et al. Evidence for the presence of a proton pump of the vacuolar H+-ATPase type in the ruffled borders of osteoclastsJ Cell BiolYear: 1990111130513112144003|
|4.||Teitelbaum SL,Ross FP. Genetic regulation of osteoclast development and functionNat Rev GenetYear: 20034:63864912897775|
|5.||Treuhaft PS. DJ MC. Synovial fluid pH, lactate, oxygen and carbon dioxide partial pressure in various joint diseasesArthritis RheumYear: 197114:4754845564921|
|6.||Semenza GL. Oxygen-dependent regulation of mitochondrial respiration by hypoxia-inducible factor 1Biochem JYear: 2007405:1917555402|
|7.||Majmundar AJ,Wong WHJ,Simon MC. Hypoxia-inducible factors and the response to hypoxic stressMol CellYear: 201040:29430920965423|
|8.||Fukuda R,Zhang HF,Kim JW,et al. HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cellsCellYear: 2007129:11112217418790|
|9.||Seagroves TN,Ryan HE,Lu H,et al. Transcription factor HIF-1 is a necessary mediator of the pasteur effect in mammalian cellsMol Cell BiolYear: 200121:3436344411313469|
|10.||Papandreou I,Cairns RA,Fontana L,et al. HIF-1 mediates adaptation to hypoxia by actively downregulating mitochondrial oxygen consumptionCell MetabYear: 20063:18719716517406|
|11.||Kim JW,Tchernyshyov I,Semenza GL,et al. HIF-1-mediated expression of pyruvate dehydrogenase kinase: a metabolic switch required for cellular adaptation to hypoxiaCell MetabYear: 20063:17718516517405|
|12.||Zhang H,Bosch-Marce M,Shimoda LA,et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxiaJ Biol ChemYear: 2008283:108921090318281291|
|13.||Arnett TR,Gibbons DC,Utting JC,et al. Hypoxia is a major stimulator of osteoclast formation and bone resorptionJ Cell PhysiolYear: 2003196:2812767036|
|14.||Knowles HJ,Athanasou NA. Acute hypoxia and osteoclast activity: a balance between enhanced resorption and increased apoptosisJ PatholYear: 2009218:25626419291710|
|15.||Muzylak M,Price JS,Horton MA. Hypoxia induces giant osteoclast formation and extensive bone resorption in the catCalcif Tissue IntYear: 200679:30130917048066|
|16.||Utting JC,Flanagan AM,Brandao-Burch A,et al. Hypoxia stimulates osteoclast formation from human peripheral bloodCell Biochem FunctYear: 201028:37438020556743|
|17.||Knowles HJ,Cleton-Jansen AM,Korsching E,et al. Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4FASEB JYear: 201024:4648465920667978|
|18.||Czupalla C,Mansukoski H,Pursche T,et al. Comparative study of protein and mRNA expression during osteoclastogenesisProteomicsYear: 20055:3868387516145714|
|19.||Kim JM,Jeong D,Kang HK,et al. Osteoclast precursors display dynamic metabolic shifts toward accelerated glucose metabolism at an early stage of RANKL-stimulated osteoclast differentiationCell Physiol BiochemYear: 200720:93594617982276|
|20.||Ishii KA,Fumoto T,Iwai K,et al. Coordination of PGC-1β and iron uptake in mitochondrial biogenesis and osteoclast activationNat MedYear: 200915:25926619252502|
|21.||Williams JP,Blair HC,McDonald JM,et al. Regulation of osteoclastic bone resorption by glucoseBiochem Biophys Res CommunYear: 1997235:6466519207213|
|22.||Larsen KI,Falany M,Wang W,et al. Glucose is a key metabolic regulator of osteoclasts; glucose stimulated increases in ATP:ADP ratio and calmodulin kinase II activityBiochem Cell BiolYear: 200583:66767316234856|
|23.||Dodds RA,Gowen M,Bradbeer JN. Microcytophotometric analysis of human osteoclast metabolism: lack of activity in certain oxidative pathways indicates inability to sustain biosynthesis during resorptionJ Histochem CytochemYear: 199442:5996068157931|
|24.||Hynes J,O'Riordan TC,Zhdanov AV,et al. In vitro analysis of cell metabolism using a long-decay pH-sensitive lanthanide probe and extracellular acidification assayAnal BiochemYear: 2009390212819379702|
|25.||Xu W,Koeck T,Lara AR,et al. Alterations of cellular bioenergetics in pulmonary artery endothelial cellsProc Natl Acad Sci USAYear: 2007104:1342134717227868|
|26.||Laderoute KR,Amin K,Calaoagan JM,et al. 5′-AMP-activated protein kinase (AMPK) is induced by low-oxygen and glucose deprivation conditions found in solid-tumor microenvironmentsMol Cell BiolYear: 200626:5336534716809770|
|27.||Huss JM,Levy FH,Kelly DP. Hypoxia inhibits the peroxisome proliferator-activated receptor-α/retinoid X receptor gene regulatory pathway in cardiac myocytes: a mechanism for O2-dependent modulation of mitochondrial fatty acid oxidationJ Biol ChemYear: 2001276:276052761211371554|
|28.||Krishnan J,Danzer C,Simka T,et al. Dietary obesity-associated Hif1α activation in adipocytes restricts fatty acid oxidation and energy expenditure via suppression of the Sirt2–NAD+ systemGenes DevYear: 201226:25927022302938|
|29.||Larsen KI,Falany ML,Ponomareva LV,et al. Glucose-dependent regulation of osteoclast H+-ATPase expression: potential role of p38 MAP-kinaseJ Cell BiochemYear: 200287:758412210724|
|30.||Su Y,Zhou A,Al-Lamki RS,et al. The α-subunit of the V-type H+-ATPase interacts with phosphofructokinase-1 in humansJ Biol ChemYear: 2003278:200132001812649290|
|31.||Lu M,Holliday LS,Zhang L,et al. Interaction between aldolase and vacuolar H+-ATPase: evidence for direct coupling of glycolysis to the ATP-hydrolyzing proton pumpJ Biol ChemYear: 2001276:304073041311399750|
|32.||Aoki J,Watanabe H,Shinozaki T,et al. FDG PET of primary benign and malignant bone tumors: standardized uptake value in 52 lesionsRadiologyYear: 2001219:77477711376267|
|33.||Strauss LG,Dimitrakopoulou-Strauss A,Koczan D,et al. 18F-FDG kinetics and gene expression in giant cell tumorsJ Nucl MedYear: 200445:1528153515347720|
|34.||Li B,Yu S. Genistein prevents bone resorption diseases by inhibiting bone resorption and stimulating bone formationBiol Pharmaceut BullYear: 200326:780786|
|35.||El Hajj Dib I,Gallet M,Mentaverri R,et al. Imatinib mesylate (Gleevec) enhances mature osteoclast apoptosis and suppresses osteoclast bone resorbing activityEur J PharmacolYear: 2006551:273317049513|
|36.||Pereira I,Fialho S,Castro G,et al. Imatinib mesylate induces clinical remission in rheumatoid arthritisJoint Bone SpineYear: 201077:37237320478730|
|37.||Soh H,Wasa M,Fukuzawa M. Hypoxia upregulates amino acid transport in a human neuroblastoma cell lineJ Pediatr SurgYear: 200742:60861217448754|
|38.||Metallo CM,Gameiro PA,Bell EL,et al. Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxiaNatureYear: 2012481:38038422101433|
|39.||Vaaraniemi J,Halleen JM,Kaarlonen K,et al. Intracellular machinery for matrix degradation in bone-resorbing osteoclastsJ Bone Miner ResYear: 200419:1432144015312243|
|40.||Bax BE,Alam AS,Banerji B,et al. Stimulation of osteoclastic bone resorption by hydrogen peroxideBiochem Biophys Res CommunYear: 1992183:115311581567393|
|41.||Koh JM,Lee YS,Kim YS,et al. Homocysteine enhances bone resorption by stimulation of osteoclast formation and activity through increased intracellular ROS generationJ Bone Miner ResYear: 200621:1003101116813521|
|42.||Srinivasan S,Koenigstein A,Joseph J,et al. Role of mitochondrial reactive oxygen species in osteoclast differentiationAnn NY Acad Sci USAYear: 20101192:245252|
|43.||Houten SM,Chegary M,Te Brinke H,et al. Pyruvate dehydrogenase kinase 4 expression is synergistically induced by AMP-activated protein kinase and fatty acidsCell Mol Life SciYear: 200966:1283129419224132|
|44.||Mungai PT,Waypa GB,Jairaman A,et al. Hypoxia triggers AMPK activation through reactive oxygen species-mediated activation of calcium release-activated calcium channelsMol Cell BiolYear: 201131:3531354521670147|
|45.||Lee YS,Kim YS,Lee SY,et al. AMP kinase acts as a negative regulator of RANKL in the differentiation of osteoclastsBoneYear: 201047:92693720696287|
The following supplementary material may be found in the online version of this article:
Additional methodology for O2 consumption assayClick here for additional data file (path0229-0755-SD1.doc)
Keywords: hypoxia, osteoclast, hypoxia-inducible factor, ATP, metabolism.
Previous Document: High-Yield Synthesis of Silver Nanoclusters Protected by DNA Monomers and DFT Prediction of their Ph...
Next Document: The effect of bacterial signal indole on the electrical properties of lipid membranes.