| Differential recovery of multimodal MRI and behavior after transient focal cerebral ischemia in rats. | |
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MedLine Citation:
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PMID: 16538230 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The association between recovery of brain function and behavior after transient cerebral ischemia in animals and humans is incompletely characterized. Quantitative diffusion- (DWI), perfusion- (PWI), T(2)-weighted (T(2)WI), and functional magnetic resonance imaging (fMRI) were performed before, during, and up to 1 day after 20-mins transient middle cerebral artery occlusion (tMCAO; n=6) or sham operation (n=6) in male Sprague-Dawley rats. Viability thresholds were employed to calculate diffusion, perfusion, and T(2) lesion volumes. Region of interest analysis was used to evaluate structural and functional MR signal changes within the sensorimotor network, which were then related to corresponding behavioral measures. Post-mortem 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed 24 h after ischemia. Transient middle cerebral artery occlusion produced lesions on DWI and PWI, which fully recovered by 30 mins after reperfusion. Ipsilesional fMRI responses to hypercapnia and forepaw stimulation were significantly impaired after ischemia and did not fully normalize until 3 and 24 h after tMCAO, respectively. No abnormalities were observed on imaging or TTC at 24 h despite significant behavioral dysfunctions including contralesional forelimb impairment and ipsilesional neglect. No MRI, behavioral, or TTC anomalies were observed in sham-operated rats. There were no significant correlations between MRI parameters, behavior, and TTC in either group. Together, these results suggest that normal findings on diffusion, perfusion, and T(2) imaging shortly after transient ischemia may not indicate normal tissue status as indicated by fMRI and behavior, which may help explain the persistence of neurologic deficits in patients with normal conventional MRI after cerebral ischemia. |
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Authors:
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Kenneth M Sicard; Nils Henninger; Marc Fisher; Timothy Q Duong; Craig F Ferris |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-03-15 |
Journal Detail:
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Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 26 ISSN: 0271-678X ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-24 Completed Date: 2006-11-14 Revised Date: 2011-06-13 |
Medline Journal Info:
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Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: United States |
Other Details:
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Languages: eng Pagination: 1451-62 Citation Subset: IM |
Affiliation:
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Department of Psychiatry, Center for Comparative NeuroImaging, University of Massachusetts Medical School, Worcester, Massachusetts 01604, USA. kenneth.sicard@umassmed.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Behavior, Animal / physiology* Brain Mapping Carbon Dioxide / blood Hypercapnia / physiopathology Ischemic Attack, Transient / pathology*, psychology* Magnetic Resonance Imaging Male Motor Activity / physiology Nerve Net / pathology, physiopathology Oxygen / blood Psychomotor Performance / physiology Rats Rats, Sprague-Dawley Sensation / physiology |
| Grant Support | |
ID/Acronym/Agency:
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R01 NS045879-01A1/NS/NINDS NIH HHS; R01 NS045879-02/NS/NINDS NIH HHS; R01 NS045879-03/NS/NINDS NIH HHS; R01 NS045879-04/NS/NINDS NIH HHS; R01 NS045879-05/NS/NINDS NIH HHS; R01 NS045879-06/NS/NINDS NIH HHS; R01-DA01915802/DA/NIDA NIH HHS; R01-NS45879/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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124-38-9/Carbon Dioxide; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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