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Differential proteomic profiling in human spermatozoa that did or did not result in pregnancy via IVF and AID.
MedLine Citation:
PMID:  24115602     Owner:  NLM     Status:  Publisher    
PURPOSE: To identify biochemical markers in men with idiopathic infertility and normal sperm counts.
EXPERIMENTAL DESIGN: We obtained proteomic profiling proteins in human spermatozoa following successful or unsuccessful pregnancy via assisted reproductive technology (ART) using 6-plex tandem mass tag (TMT) isobaric mass spectrometry. Our study design consisted of two groups: 1. The semen of 6 men whose sperm resulted in a clinical pregnancy following ART and 6 men whose semen did not result in a clinical pregnancy following ART. The results of differentiated mass spectrometry were validated by Western blotting.
RESULTS AND DISCUSSION: A total of 2,045 proteins were detected in our cohort. 21 proteins were found to be differentially expressed (>1.2-fold) in men whose sperm resulted in a clinical pregnancy and those that did not. Using the results of bioinformatics analysis and Western Blotting, three proteins (A2LD1, ATP1B3 and FBXO2) were shown to have the same differential pattern (p<0.05) that was observed in the mass spectrometry analysis.
CONCLUSIONS AND CLINICAL RELEVANCE: Proteomics may help identity a select cohort of men with abnormal semen parameters and aide infertility diagnoses.
Yong Zhu; Yibo Wu; Ke Jin; Hui Lu; Feng Liu; Yueshuai Guo; Feng Yan; Wenbo Shi; Yong Liu; Xiaorong Cao; Hongliang Hu; Hui Zhu; Xuejiang Guo; Jiahao Sha; Zheng Li; Zuoming Zhou
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-10-1
Journal Detail:
Title:  Proteomics. Clinical applications     Volume:  -     ISSN:  1862-8354     ISO Abbreviation:  Proteomics Clin Appl     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101298608     Medline TA:  Proteomics Clin Appl     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Renji Hospital, Department of Urology, Sperm Development and Genetics Laboratory, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200001, P. R. China.
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