Document Detail


Differential proteomic analysis of the anti-proliferative effect of glucocorticoid hormones in ST1 rat glioma cells.
MedLine Citation:
PMID:  17127050     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glucocorticoid hormones (GCs) exert a potent anti-proliferative activity on several cell types. The classic molecular mechanism of GCs involves modulation of the activity of the glucocorticoids receptor, a transcriptional regulator. However, the anti-proliferative effect of GCs may also involve modulation of processes such as translation, subcellular localization and post-translational modifications, which are not reflected at the mRNA level. To investigate these potential effects of GCs, we employed the proteomic approach (two-dimensional electrophoresis and mass spectrometry) and the ST1 cells, obtained from the C6 rat glioma cell line, as a model. GC treatment leads ST1 cells to a complete transformed-to-normal phenotypic reversion and loss of their tumorigenic potential. By comparing sets of 2D nuclear protein profiles of ST1 cells treated (or not) with hydrocortisone (Hy), 13 polypeptides displaying >or=two-fold difference in abundance upon Hy treatment were found. Five of these polypeptides were identified by peptide mass fingerprinting, including Annexin 2 (ANX2), hnRNP A3 and Ubiquitin. Evidence obtained by Western blot analysis indicates that ANX2 is present in the nucleus and has its subcellular localization modulated by GC-treatment of ST1 cells. Our findings indicate complementary mechanisms contributing to the regulation of gene expression associated with ST1 cells' response to GCs.
Authors:
Marcos A A Demasi; Wagner R Montor; Gabriela B Ferreira; Daniel C Pimenta; Letícia Labriola; Mari C Sogayar
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-28
Journal Detail:
Title:  The Journal of steroid biochemistry and molecular biology     Volume:  103     ISSN:  0960-0760     ISO Abbreviation:  J. Steroid Biochem. Mol. Biol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-29     Completed Date:  2007-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015483     Medline TA:  J Steroid Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  137-48     Citation Subset:  IM    
Affiliation:
Instituto de Química, Universidade de São Paulo, 05508-900 SP, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexin A2 / analysis,  metabolism
Cell Proliferation / drug effects*
Glioma / pathology*
Glucocorticoids / pharmacology*
Hydrocortisone / pharmacology
Mass Spectrometry
Nuclear Proteins / analysis
Peptide Mapping / methods
Proteomics*
Rats
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Annexin A2; 0/Glucocorticoids; 0/Nuclear Proteins; 50-23-7/Hydrocortisone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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