Document Detail


Differential proteomic analysis of HeLa cells treated with Honokiol using a quantitative proteomic strategy.
MedLine Citation:
PMID:  18157584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Honokiol (HNK) is an active component purified from Magnolia officinalis. HNK exhibits antitumor effects by inducing apoptosis and inhibiting the growth of many cancer cell lines, while proteins involved in antitumor effects in proteomic level are still unclear. In our study, HNK could inhibit HeLa cell proliferation and induce apoptosis in a concentration- and time-dependent manner. We utilized a quantitative proteomic technique termed SILAC (Stable isotope labeling with amino acids in cell culture)-MS (mass spectrometry) to study the differential proteomic profiling of HeLa cells treated by HNK. A total of 85 proteins were changed after HeLa cells were treated with 12 microg/ml HNK for 8 h, and 8 proteins showed up-regulation while 77 proteins down-regulated. The changed proteins were classified into 9 different categories, which covered a broad variety of cellular functions. In conclusion, HNK performs cytotoxicity to HeLa cells through co-operating of many proteins and different pathways.
Authors:
B Ling; S-F Liang; Y-H Xu; X-Y Zhao; M-H Tang; X-Y Liu; X Zhao; C-H Huang; L-J Chen; Y-Q Wei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-21
Journal Detail:
Title:  Amino acids     Volume:  35     ISSN:  1438-2199     ISO Abbreviation:  Amino Acids     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-05     Completed Date:  2008-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  115-22     Citation Subset:  IM    
Affiliation:
West China Second Hospital, and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / chemistry,  pharmacology*
Apoptosis / drug effects
Biphenyl Compounds / chemistry,  pharmacology*
Cell Proliferation / drug effects
Cytotoxins / chemistry,  pharmacology*
Dose-Response Relationship, Drug
Gene Expression Regulation, Neoplastic / drug effects*
Hela Cells
Humans
Lignans / chemistry,  pharmacology*
Magnolia / chemistry
Neoplasm Proteins / biosynthesis*
Proteome / metabolism*
Proteomics / methods
Time Factors
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Biphenyl Compounds; 0/Cytotoxins; 0/Lignans; 0/Neoplasm Proteins; 0/Proteome; 35354-74-6/honokiol

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