| Differential protein profiling of primary versus immortalized human RPE cells identifies expression patterns associated with cytoskeletal remodeling and cell survival. | |
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MedLine Citation:
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PMID: 16602694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Functional research of retinal pigment epithelium (RPE) most often relies on utilization of RPE-derived cell lines in vitro. However, no studies about similarities and differences of the respective cell lines exist so far. Thus, we here analyze the proteome of the most popular RPE cell lines: ARPE-19 and hTERT and compare their constitutive and de novo synthesized protein expression profiles to human early passage retinal pigment epithelial cells (epRPE) by 2-D electrophoresis and MALDI-TOF peptide mass fingerprinting. In all three cell lines the baseline protein expression pattern corresponded well to the de novo synthesized cellular proteome. However, comparison of the protein profile of epRPE cells with that of hTERT-RPE cells revealed a higher abundance of proteins related to cell migration, adhesion, and extracellular matrix formation, paralleled by a down-regulation of proteins attributed to cell polarization, and showed an altered expression of detoxification enzymes in hTERT-RPE. ARPE-19 cells, however, exhibited a higher abundance of components of the microtubule cytoskeleton and differences in expression of proteins related to proliferation and cell death. epRPE cells, hTERT-RPE, and ARPE-19 therefore may respond differently with respect to certain functional properties, a finding that should prove valuable for future in vitro studies. |
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Authors:
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Claudia S Alge; Stefanie M Hauck; Siegfried G Priglinger; Anselm Kampik; Marius Ueffing |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of proteome research Volume: 5 ISSN: 1535-3893 ISO Abbreviation: J. Proteome Res. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-10 Completed Date: 2006-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 862-78 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany, and GSF Research Center for Environment and Health, Neuherberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques Cell Line Cell Line, Transformed Cell Survival Cells, Cultured Cytoskeletal Proteins / metabolism* Databases, Protein Electrophoresis, Gel, Two-Dimensional Humans Peptide Mapping Pigment Epithelium of Eye / cytology*, metabolism* Protein Array Analysis / methods* Proteome / analysis Proteomics / methods* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tubulin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cytoskeletal Proteins; 0/Proteome; 0/Tubulin |
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