Document Detail


Differential protein insertion into developing photosynthetic membrane regions of Rhodopseudomonas sphaeroides.
MedLine Citation:
PMID:  6609927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies have suggested that much of the B800-850 light-harvesting bacteriochlorophyll a-protein complex is inserted directly into the intracytoplasmic photosynthetic membrane of Rhodopseudomonas sphaeroides. In contrast, the B875 light-harvesting and reaction center complexes are assembled preferentially at peripheral sites of photosynthetic membrane growth initiation. The basis for this apparent site-specific polypeptide insertion was examined during the inhibition of RNA and protein syntheses. The pulse labeling of polypeptides at the membrane growth initiation sites was significantly less sensitive to inhibition by rifampicin, chloramphenicol, or kasugamycin than in the intracytoplasmic or outer membranes. This suggests increased stability for the translation machinery at these membrane invagination sites. Similar differential effects in polypeptide insertion were observed during inhibition of bacteriochlorophyll synthesis through deprival of delta-aminolevulinate to R sphaeroides mutant H-5, which requires this porphyrin precursor. The pulse-labeling patterns observed during the inhibition of both RNA and pigment syntheses were consistent with the uncoupling of polypeptide insertion into the membrane invagination sites from their growth and maturation into intracytoplasmic membranes.
Authors:
G S Inamine; P A Reilly; R A Niederman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  24     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-10     Completed Date:  1984-07-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  69-77     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology
Bacterial Chromatophores / metabolism
Bacterial Proteins / metabolism*
Cell Membrane / metabolism
Intracellular Membranes / metabolism
Membrane Proteins / metabolism
Photosynthesis
Rhodobacter sphaeroides / drug effects,  metabolism*
Grant Support
ID/Acronym/Agency:
GM26248-04/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Membrane Proteins; 0/bacteriochlorophyll A proteins, Bacteria

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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