Document Detail


Differential proliferation dependence of alpha and beta damage in X-irradiated Chinese hamster cells.
MedLine Citation:
PMID:  10356614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To determine quantitatively the influence of altering proliferation rates on clonal survival of asynchronously growing Chinese hamster (CHO) cells after X-irradiation and to evaluate the related contribution of alpha and beta damage. MATERIAL AND METHODS: Cell cycle distributions at the time of X-irradiation of CHO cells were assessed by flow cytometry. Clonal radiation survival was established by colony forming assay. Survival data were fitted to the linear-quadratic model and analyzed on the basis of the mean inactivation dose, D. RESULTS: Increased S-phases were associated with increased resistance to X-rays. Radiosensitivity as expressed by D differed by a factor of 1.6 between the most sensitive and the most resistant populations. Separately analyzing the alpha and beta coefficients of survival curves revealed that the proliferation dependent effect was correlated only with beta. The major determinant of D was alpha, but its substantial interexperimental variations were independent of the cell cycle. CONCLUSIONS: Due to altering cell cycle distributions, considerable changes of radiosensitivity can occur. They can in part be understood as a consequence of S-phase dependent alterations of DNA damage repair. Reasons for the changes of a damage dependent lethality remain to be discovered by further research.
Authors:
D Bartkowiak; W Nothdurft; E M Röttinger
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]     Volume:  175     ISSN:  0179-7158     ISO Abbreviation:  Strahlenther Onkol     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-06-18     Completed Date:  1999-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603469     Medline TA:  Strahlenther Onkol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  239-44     Citation Subset:  IM    
Affiliation:
Abteilung für Strahlentherapie, Universität Ulm. detlef.bartkowiak@medizin.uni-ulm.de
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells / cytology,  radiation effects*
Cell Count
Cell Cycle
Cell Division
Cricetinae
DNA / radiation effects*
DNA Repair*
Data Interpretation, Statistical
Dose-Response Relationship, Radiation
Flow Cytometry
Linear Models
Radiation Dosage
Radiation Tolerance*
S Phase
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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