Document Detail


Differential production of adrenal steroids by purified cells of the human adrenal cortex is relative rather than absolute.
MedLine Citation:
PMID:  12534367     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The adrenal cortex produces aldosterone, cortisol and androgens in response to ACTH and angiotensin II. To define the differential response of morphologically distinct cells of the adrenal cortex, we examined the phenotypical and functional characteristics of human adrenocortical cells. RESULTS: Tumour growth factor-beta receptor-1 (TGFbeta-R1) and CYP-11 were found to be expressed predominantly in the zona fasciculata, whereas human leukocyte antigen (HLA-DR) and CYP-17 were localised to the zona reticularis. The angiotensin II receptor, AT-1, was found to be predominantly expressed in the zona glomerulosa. Adrenocortical cells, separated by density, yielded two distinct fractions which displayed differential growth patterns. Lipid-rich cells of fraction I expressed TGFbeta-R1 and produced significantly more cortisol relative to androstenedione than unseparated or fraction II cells, whereas lipid-poor cells of fraction II expressed HLA-DR and produced more androstenedione relative to cortisol in the presence of ACTH. Aldosterone production by fraction II was significantly greater than fraction I or unseparated cells. TGFbeta-R1-positive fasciculata-type cells separated into fraction I and HLA-DR-positive cells consistent with reticularis cells separated into fraction II. Aldosterone-producing cells indicative of glomerulosa cells separated into fraction II. CONCLUSIONS: Our findings are consistent with the concept that all adrenocortical cells are capable of producing a range of steroids, but the relative production of cortisol, androgen and aldosterone differs.
Authors:
Leonie S Young; Geraldine Murphy; Sinead N Kelly; Thomas P Smith; Sean K Cunningham; T Joseph McKenna
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of endocrinology / European Federation of Endocrine Societies     Volume:  148     ISSN:  0804-4643     ISO Abbreviation:  Eur. J. Endocrinol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-01-21     Completed Date:  2003-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423848     Medline TA:  Eur J Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  139-45     Citation Subset:  IM    
Affiliation:
Department of Investigative Endocrinology, St Vincent's University Hospital and the Conway Institute, University College, Dublin, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Cortex / chemistry,  cytology*,  metabolism*
Adrenocorticotropic Hormone / pharmacology
Aldosterone / analysis,  biosynthesis
Androgens / analysis,  biosynthesis
Angiotensin II / pharmacology
Biological Markers
Cell Differentiation / drug effects,  physiology
Cells, Cultured
Humans
Hydrocortisone / analysis,  biosynthesis
Immunohistochemistry
Vasoconstrictor Agents / pharmacology
Chemical
Reg. No./Substance:
0/Androgens; 0/Biological Markers; 0/Vasoconstrictor Agents; 11128-99-7/Angiotensin II; 50-23-7/Hydrocortisone; 52-39-1/Aldosterone; 9002-60-2/Adrenocorticotropic Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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