Document Detail

Differential processing of the two subunits of human choriogonadotropin by granulosa cells. II. In vivo studies.
MedLine Citation:
PMID:  6273119     Owner:  NLM     Status:  MEDLINE    
Using a well characterized preparation of hCG, consisting of a mixture of hCG labeled in the alpha-subunit with 125I and hCG labeled in the beta-subunit with 131I (see preceding paper), the hormone-specific preferential retention by granulosa cells in vivo of the radiolabel originally associated with the beta-subunit of hCG has been confirmed and extended. Additional studies have shown that this retention is peculiar to the granulosa cells. Luteal and interstitial/thecal elements of the ovary failed to show preferential accumulation of the label originally associated with the beta-subunit. Measurement of both radioactivities in crude subfractions of the ovarian tissues revealed that granulosa cells retain the excess of beta-subunit label in a plasma membrane/vesicular component. No such preferential retention of label was seen in any of the subfractions obtained from luteal or interstitial/thecal tissues. The radiolabeled components associated with the granulosa cells were shown to be mainly macromolecular by their precipitability with 13% trichloroacetic acid. Luteal tissue degraded the components associated with each label more rapidly than granulosa cells. In contrast, interstitial/thecal tissue degraded very little of the bound labeled components. The differential processing of individual hCG subunits by granulosa cells was shown not to result from different kinetics of binding of serum-borne hormone by two methods. Thus, changes over time in the ability of circulating hormone to bind to LH receptor in vitro were shown not to be a function of the hCG subunit having the label. Moreover, blockade of further radiolabel uptake by injection of a large excess of unlabeled hCG 30 min after radiolabel administration did not alter the rise in the ratio of beta-subunit label to alpha-subunit label normally observed in granulosa cells. The ability of kidney tissue to accumulate and metabolize hCG also varied with the physiological state. Within the limitations of following the radioiodides added to proteins rather than the peptides themselves, these studies demonstrate that differences exist in the metabolism of hCG by the various target cells of the ovary and that changes in processing occur during luteinization.
K L Campbell; P Bagavandoss; M D Byrne; J A Jonassen; T D Landefeld; M W Quasney; M M Sanders; A R Midgley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  109     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-02-22     Completed Date:  1982-02-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1858-71     Citation Subset:  AIM; IM    
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MeSH Terms
Chorionic Gonadotropin / metabolism*
Estradiol / pharmacology
Follicle Stimulating Hormone / pharmacology
Granulosa Cells / drug effects,  metabolism*
Iodine Radioisotopes
Kidney / metabolism
Macromolecular Substances
Ovarian Follicle / metabolism
Ovary / metabolism*
Pseudopregnancy / metabolism
Radioisotope Dilution Technique
Receptors, Cell Surface / drug effects,  metabolism*
Receptors, LH
Grant Support
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Iodine Radioisotopes; 0/Macromolecular Substances; 0/Receptors, Cell Surface; 0/Receptors, LH; 50-28-2/Estradiol; 9002-68-0/Follicle Stimulating Hormone

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