Document Detail

Differential participation of homologous recombination and nucleotide excision repair in yeast survival to ultraviolet light radiation.
MedLine Citation:
PMID:  20348017     Owner:  NLM     Status:  MEDLINE    
AIMS: The purpose of this research was to assess the ultraviolet light (UV) phenotype of yeast sirDelta cells vs. WT cells, and to determine whether de-silenced chromatin or the intrinsic pseudoploidy of sirDelta mutants contributes to their response to UV. Additional aims were to study the participation of HR and NER in promoting UV survival during the cell cycle, and to define the extent of the co-participation for both repair pathways. MAIN METHODS: The sensitivity of yeast Saccharomyces cerevisiae to UV light was determined using a method based on automatic measurements of optical densities of very small (100mul) liquid cell cultures. KEY FINDINGS: We show that pseudo-diploidy of sirDelta strains promotes resistance to UV irradiation and that HR is the main mechanism that is responsible for this phenotype. In addition, HR together with GG-NER renders cells in the G2-phase of the cell cycle more resistant to UV irradiation than cells in the G1-phase, which underscore the importance of HR when two copies of the chromosomes are present. Nevertheless, in asynchronously growing cells NER is the main repair pathway that responds to UV induced DNA damage. SIGNIFICANCE: This study provides detailed and quantitative information on the co-participation of HR and NER in UV survival of yeast cells.
Martin Toussaint; Raymund J Wellinger; Antonio Conconi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-27
Journal Detail:
Title:  Mutation research     Volume:  698     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-27     Completed Date:  2010-07-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  52-9     Citation Subset:  IM    
Copyright Information:
Crown Copyright 2010. Published by Elsevier B.V. All rights reserved.
Département de Microbiologie et d'Infectiologie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada.
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MeSH Terms
Base Sequence
Cell Cycle
Cell Survival
DNA Damage
DNA Repair*
Molecular Sequence Data
Recombination, Genetic*
Saccharomyces cerevisiae / genetics*,  radiation effects*
Silent Information Regulator Proteins, Saccharomyces cerevisiae / genetics*
Sirtuin 2 / genetics*
Time Factors
Ultraviolet Rays / adverse effects*
Grant Support
MOP 12616//Canadian Institutes of Health Research
Reg. No./Substance:
0/Silent Information Regulator Proteins, Saccharomyces cerevisiae; EC 3.5.1.-/SIR2 protein, S cerevisiae; EC 3.5.1.-/Sirtuin 2

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