Document Detail

Differential pH in embryo culture.
MedLine Citation:
PMID:  21067724     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine the optimum pH in sequential media for embryo culture around the fertilization-zygote stage and cleavage stage, with use of a mouse embryo assay.
DESIGN: Experimental laboratory study.
SETTING: University Hospital and University Research Unit.
ANIMAL(S): F1 hybrids between CD1 female and BDF male mice.
INTERVENTION(S): Fertilized, one-cell mouse embryos were cultured 5 days in test media where pH was changed at defined time intervals.
MAIN OUTCOME MEASURE(S): Percentage of good-quality embryos, defined by strict morphology.
RESULT(S): A significantly improved development was observed when pH was as high as 7.30 before the pronuclear stage and lowered to pH 7.15 during the cleavage period.
CONCLUSION(S): Good embryo development is consistent with two different pH values in sequential culture media. This could have important implications for embryo culture in human IVF.
Martha Hentemann; Karim Mousavi; Kjell Bertheussen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-10
Journal Detail:
Title:  Fertility and sterility     Volume:  95     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-07     Completed Date:  2011-05-12     Revised Date:  2011-08-12    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1291-4     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Department of Obstetrics and Gynecology, IVF Unit, University Hospital of Northern Norway, Tromsø, Norway.
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MeSH Terms
Cells, Cultured
Culture Media / chemistry*,  pharmacology
Embryonic Development / drug effects,  physiology*
Hydrogen-Ion Concentration
Oocytes / cytology*,  drug effects,  physiology*
Reg. No./Substance:
0/Culture Media
Comment In:
Fertil Steril. 2011 Jun 30;95(8):e67; author reply e68   [PMID:  21536276 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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