| Differential modulation of apoptotic gene expression by NAC in Leydig cells stimulated persistently with hCG in vivo. | |
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MedLine Citation:
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PMID: 21856376 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The present study was designed to investigate the molecular mechanisms of NAC (150mg/kg bw twice/week) action in vivo under repeated hCG (100IU/rat/day) stimulation to adult rats. Leydig cell refractoriness led to a significant decline in serum testosterone and intracellular cAMP by day 30 of chronic hCG intervention which improved significantly following NAC co-administration. It inhibited the rise in lipid peroxidation, improved the activity of antioxidant enzymes along with intracellular glutathione and total antioxidant capacity in the target cells. Leydig cell apoptosis declined significantly (P<0.001) with down-regulation of upstream, Fas, FasL, caspase-8, Bax and caspase-9, JNK/pJNK and downstream caspase-3 and PARP. On the other hand, anti-apoptotic Bcl2, NF-kβ, and Akt were up-regulated. Taken together, the above findings indicate that the specificity of NAC action was not restricted to regulating marker proteins in the extrinsic and JNK pathways as seen in vitro but extended to include intrinsic pathway of metazoan apoptosis as well. |
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Authors:
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Archana Aggarwal; M M Misro; Ankur Maheshwari; Neeta Sehgal |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-12 |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: - ISSN: 1872-8057 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-8-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, Baba Gang Nath Marg, Munirka, New Delhi 110067, India; Department of Zoology, University of Delhi, Delhi 110007, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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