| Differential methylation of STOX1 in human placenta. | |
| | |
MedLine Citation:
|
PMID: 20716964 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
The 10q22 chromosomal region with genomic linkage to pre-eclampsia in Dutch females shows a parent-of-origin effect with maternal transmission of the Y153H susceptibility allele of the STOX1 gene. Although the CpG island within the STOX1 promoter region shows no differential methylation, this study describes the identification of a differentially methylated region (DMR) in intron 1 of the STOX1 gene. Methylation coincides with STOX1 expression, where high methylation leads to reduced expression. In the SGHPL-5 extravillous trophoblast cell line allele-specific expression was observed in a subset of cells. Although no allele-specific expression could be detected in early placenta samples, these samples did show an increase in methylation when they were homozygous for the Y153H susceptibility allele. Allele-specific methylation was observed in column extravillous trophoblast samples with the methylated allele being paternal in origin. We conclude that STOX1 is paternally imprinted, maternally expressed, with the DMR identified in this study showing parental-specific methylation in specific cell-types, hypothesized to occur in villous cytotrophoblasts, and proven in column extravillous trophoblasts originating from the anchoring villus. In other (placental) cells methylation is independent of parental origin, but regulates STOX1 expression with the Y153H genotype directing the level of methylation. |
| | |
Authors:
|
Marie van Dijk; Sascha Drewlo; Cees B M Oudejans |
Related Documents
:
|
12579314 - Promoter hypermethylation of multiple genes in astrocytic gliomas. 16343074 - Methylation status of p14arf, p15ink4b, and p16ink4a genes in human hepatocellular carc... 12935354 - Bacterial lps and cpg dna differentially induce gene expression profiles in mouse macro... 16352824 - Identification and characterization of the genes involved in glycosylation pathways of ... 10599704 - Sibling pair linkage and association studies between bone mineral density and the insul... 11118044 - Identification of differentially expressed genes in human gliomas by dna microarray and... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-01 |
Journal Detail:
|
Title: Epigenetics : official journal of the DNA Methylation Society Volume: 5 ISSN: 1559-2308 ISO Abbreviation: Epigenetics Publication Date: 2010 Nov-Dec |
Date Detail:
|
Created Date: 2010-12-02 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101265293 Medline TA: Epigenetics Country: United States |
Other Details:
|
Languages: eng Pagination: 736-42 Citation Subset: IM |
Affiliation:
|
Department of Clinical Chemistry, VU University Medical Center, Amsterdam, The Netherlands. m.vdijk@vumc.nl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
MGC-13299//Canadian Institutes of Health Research |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Disrupted microRNA expression caused by Mecp2 loss in a mouse model of Rett syndrome.
Next Document: Quantitative analysis of promoter methylation in exfoliated epithelial cells isolated from breast mi...