Document Detail

Differential location of alpha-expansin proteins during the accommodation of root cells to an arbuscular mycorrhizal fungus.
MedLine Citation:
PMID:  15605243     Owner:  NLM     Status:  MEDLINE    
alpha-Expansins are extracellular proteins that increase plant cell-wall extensibility. We analysed their pattern of expression in cucumber roots in the presence and in the absence of the mycorrhizal fungus, Glomus versiforme. The distribution of alpha-expansins was investigated by use of two polyclonal antibodies (anti-EXPA1 and anti-EXPA2, prepared against two different cucumber alpha-expansins) in immunoblotting, immunofluorescence, and immunogold experiments. Immunoblot results indicate the presence of a 30-kDa band specific for mycorrhizal roots. The two antibodies identify antigens with a different distribution in mycorrhizal roots: anti-EXPA1 labels the interface zone, but the plant cell walls only weakly. By contrast, the anti-EXPA2 labels only the plant cell walls. In order to understand the potential role of alpha-expansins during the accommodation of the fungus inside root cells, we prepared semi-thin sections to measure the size of cortical cells and the thickness of cortical cell walls in mycorrhizal and non-mycorrhizal root. Mycorrhizal cortical cells were significantly larger than non-mycorrhizal cells and had thicker cell walls. In double-labelling experiments with cellobiohydrolase-gold complex, we observed that cellulose was co-localized with alpha-expansins. Taken together, the results demonstrate that alpha-expansins are more abundant in the cucumber cell walls upon mycorrhizal infection; we propose that these wall-loosening proteins are directly involved in the accommodation of the fungus by infected cortical cells.
R Balestrini; D J Cosgrove; P Bonfante
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-17
Journal Detail:
Title:  Planta     Volume:  220     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-21     Completed Date:  2005-10-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  889-99     Citation Subset:  IM    
Istituto per la Protezione delle Piante del CNR, Sezione di Micologia and Dipartimento di Biologia Vegetale dell'Università, Viale Mattioli 25, 10125, Turin, Italy.
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MeSH Terms
Cell Wall / metabolism,  microbiology,  ultrastructure
Cellulose / metabolism
Cucumis sativus / metabolism*,  microbiology
Fluorescent Antibody Technique
Fungi / physiology*
Gene Expression
Microscopy, Electron, Transmission
Mycorrhizae / physiology*
Plant Proteins / metabolism*
Plant Roots / cytology,  microbiology*,  ultrastructure
Reg. No./Substance:
0/Plant Proteins; 0/expansin protein, plant; 9004-34-6/Cellulose

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