Document Detail


Differential localization of arabinan and galactan side chains of rhamnogalacturonan 1 in cambial derivatives.
MedLine Citation:
PMID:  10805444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of pectin structural features during the differentiation of cambial derivatives was investigated in aspen (Populus tremula L. x P. tremuloides Michx.) using biochemical and immunocytochemical methods. Comparisons were also made between active and resting tissues. Active tissues, in particular cambial cells and phloem derivatives, were characterized by a high pectin content. Use of antibodies raised against arabinan side chains of rhamnogalacturonan 1 (LM6), as well as biochemical analysis, revealed an obvious decrease from the cortex to the differentiating xylem. Galactan side chains, detected with LM5 antibodies, were present mainly in the cambial zone and enlarging xylem cells. In contrast, they were totally absent from sieve-tube cell walls. Image analysis of LM5 immunogold labelling in the cambial zone showed a clustered distribution of galactan epitopes in the radial walls, a distribution which might result from the association of two different periodic processes, namely the exocytosis of galactan and wall expansion. Cessation of cambial activity was characterized by cell wall thickening accompanied by a sharp decrease in the relative amount of pectin and a lowering of the degree of methylesterification. The data provide evidence that the walls of phloem and xylem cells differ in their pectin composition even at a very early stage of commitment. These differences offer useful tools for identifying the initial cells among their immediate neighbours.
Authors:
F F Ermel; M L Follet-Gueye; C Cibert; B Vian; C Morvan; A M Catesson; R Goldberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Planta     Volume:  210     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-07-28     Completed Date:  2000-07-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  732-40     Citation Subset:  IM; S    
Affiliation:
UMR CNRS 7592, Institut Jacques Monod, Université Paris 6, France.
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MeSH Terms
Descriptor/Qualifier:
Cell Wall / chemistry
Fluorescent Antibody Technique
Galactans / analysis*
Microscopy, Confocal
Microscopy, Immunoelectron
Pectins / analysis,  chemistry*
Plant Structures / anatomy & histology,  chemistry*,  ultrastructure
Polysaccharides / analysis*
Time Factors
Trees / chemistry,  growth & development
Chemical
Reg. No./Substance:
0/Galactans; 0/Pectins; 0/Polysaccharides; 0/rhamnogalacturonan I; 11078-27-6/araban

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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