Document Detail

Differential labelling of bulk endocytosis in nerve terminals by FM dyes.
MedLine Citation:
PMID:  18586059     Owner:  NLM     Status:  MEDLINE    
Bulk endocytosis is triggered in central nerve terminals during intense physiological stimulation. This endocytosis pathway can be labelled by the dye FM1-43 but not its more hydrophilic counterpart FM2-10. This selective labelling was proposed to be due to the retention of FM1-43, but not FM2-10, in slowly retrieving structures after washout of the dye. However, this explanation assumed that bulk endocytosis was a slow process that persisted after stimulation. We have recently shown that the great majority of bulk endocytosis occurs during stimulation, therefore another explanation for the specific labelling of this pathway by FM1-43 must be found. In this paper we show that the ability of FM dyes to label bulk endocytosis is dependent on the concentration of dye used and not their washout properties. When the loading concentration of FM1-43 was reduced 10-fold, its ability to label bulk endocytosis was lost. Conversely when the loading concentration of FM2-10 was increased 10-fold, it now labelled the pathway. This suggests that a difference in affinity of bulk endosome membranes for FM1-43 and FM2-10 underlies the disparity in labelling.
E L Clayton; M A Cousin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-11
Journal Detail:
Title:  Neurochemistry international     Volume:  53     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-08     Completed Date:  2008-11-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  51-5     Citation Subset:  IM    
Membrane Biology Group, Centre for Integrative Physiology, George Square, University of Edinburgh, Scotland, UK.
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MeSH Terms
Axonal Transport / physiology
Cells, Cultured
Central Nervous System / physiology*,  ultrastructure
Dose-Response Relationship, Drug
Endocytosis / physiology*
Endosomes / drug effects,  metabolism
Fluorescent Dyes / metabolism*,  pharmacokinetics
Intracellular Membranes / drug effects,  metabolism
Neurons / physiology,  ultrastructure
Presynaptic Terminals / physiology*,  ultrastructure
Pyridinium Compounds / metabolism,  pharmacokinetics
Quaternary Ammonium Compounds / metabolism,  pharmacokinetics
Rats, Sprague-Dawley
Staining and Labeling / methods*
Synaptic Transmission / physiology
Transport Vesicles / drug effects,  metabolism
Reg. No./Substance:
0/FM1 43; 0/FM2 10; 0/Fluorescent Dyes; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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