| Differential labeling of the subunits of respiratory complex III with [3H]succinic anhydride, [14C]succinic anhydride, and p-diazobenzene-[35S]sulfonate. | |
| | |
MedLine Citation:
|
PMID: 3007448 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Exposure of antimycin-treated Complex III (ubiquinol-cytochrome c reductase) purified from bovine heart mitochondria to [3H]succinic anhydride plus [35S]p-diazobenzenesulfonate (DABS) resulted in somewhat uniform relative labeling of the eight measured subunits of the complex by [3H]succinic anhydride. In contrast, relative labeling by [35S]DABS was similar to [3H]succinic anhydride for the subunits of high molecular mass, i.e., core proteins, cytochromes, and the iron-sulfur protein, but greatly reduced for the polypeptides of molecular mass below 15 kDa. With Complex II depleted in the iron-sulfur protein the relative labeling of core protein I by exposure of the complex to [3H]succinic anhydride was significantly enhanced, whereas labeling of the polypeptides represented by SDS-PAGE bands 7 and 8 was significantly inhibited. Dual labeling of the subunits of Complex III by 14C- and 3H-labeled succinic anhydride before and after dissociation of the complex by sodium dodecyl sulfate, respectively, was measured with the complex in its oxidized, reduced, and antimycin-inhibited states. Subunits observed to be most accessible or reactive to succinic anhydride were core protein II, the iron-sulfur protein, and polypeptides of SDS-PAGE bands 7,8, and 9. Two additional polypeptides of molecular masses 23 and 12kDa, not normally resolved by gel-electrophoresis, were detected. Reduction of the complex resulted in a significant change of 14C/3H labeling ratio of core protein only, whereas treatment of the complex with antimycin resulted in decreases in 14C/3H labeling ratios of core proteins I and II, cytochrome c1, and a polypeptide of molecular mass 13kDa identified as an antimycin-binding protein. |
| | |
Authors:
|
S H Ho; J S Rieske |
Related Documents
:
|
15143178 - The yeast scaffold proteins isu1p and isu2p are required inside mitochondria for matura... 8063708 - Mössbauer characterization of the metal clusters in azotobacter vinelandii nitrogenase... 7750738 - Utilization of hemin and hemoglobin as iron sources by vibrio parahaemolyticus and iden... 2153958 - Site-directed mutagenesis of azotobacter vinelandii ferredoxin i: [fe-s] cluster-driven... 15619298 - Comparison of different depletion strategies for improved resolution in proteomic analy... 18568658 - Rhabdomyosarcoma of the pectoral muscles of a free-living european robin (erithacus rub... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of bioenergetics and biomembranes Volume: 17 ISSN: 0145-479X ISO Abbreviation: J. Bioenerg. Biomembr. Publication Date: 1985 Dec |
Date Detail:
|
Created Date: 1986-05-05 Completed Date: 1986-05-05 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7701859 Medline TA: J Bioenerg Biomembr Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 333-48 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antimycin A / pharmacology Benzenesulfonates / metabolism* Carbon Radioisotopes Cattle Diazonium Compounds / metabolism* Electron Transport Complex III Electrophoresis, Polyacrylamide Gel Iron-Sulfur Proteins / metabolism Mitochondria, Heart / metabolism* Multienzyme Complexes / metabolism* Oxidation-Reduction Protein Conformation Quinone Reductases / metabolism* Succinates / metabolism* Succinic Anhydrides / metabolism* Sulfanilic Acids / metabolism* Tritium |
| Grant Support | |
ID/Acronym/Agency:
|
HL-265298/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Benzenesulfonates; 0/Carbon Radioisotopes; 0/Diazonium Compounds; 0/Iron-Sulfur Proteins; 0/Multienzyme Complexes; 0/Rieske iron-sulfur protein; 0/Succinates; 0/Succinic Anhydrides; 0/Sulfanilic Acids; 10028-17-8/Tritium; 2154-66-7/diazobenzenesulfonic acid; 642-15-9/Antimycin A; EC 1.10.2.2/Electron Transport Complex III; EC 1.6.99.-/Quinone Reductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Two non-identical heads of myosin molecules.
Next Document: Effect of trypsin on the kinetic properties of reconstituted beef heart cytochrome c oxidase.